Title |
The Oleaginous Yeast Meyerozyma guilliermondii BI281A as a New Potential Biodiesel Feedstock: Selection and Lipid Production Optimization
|
---|---|
Published in |
Frontiers in Microbiology, September 2017
|
DOI | 10.3389/fmicb.2017.01776 |
Pubmed ID | |
Authors |
Mauricio Ramírez-Castrillón, Victoria P. Jaramillo-Garcia, Priscila D. Rosa, Melissa F. Landell, Duong Vu, Mariana F. Fabricio, Marco A. Z. Ayub, Vincent Robert, João A. P. Henriques, Patricia Valente |
Abstract |
A high throughput screening (HTS) methodology for evaluation of cellular lipid content based on Nile red fluorescence reads using black background 96-wells test plates and a plate reader equipment allowed the rapid intracellular lipid estimation of strains from a Brazilian phylloplane yeast collection. A new oleaginous yeast, Meyerozyma guilliermondii BI281A, was selected, for which the gravimetric determination of total lipids relative to dry weight was 52.38% for glucose or 34.97% for pure glycerol. The lipid production was optimized obtaining 108 mg/L of neutral lipids using pure glycerol as carbon source, and the strain proved capable of accumulating oil using raw glycerol from a biodiesel refinery. The lipid profile showed monounsaturated fatty acids (MUFA) varying between 56 or 74% in pure or raw glycerol, respectively. M. guilliermondii BI281A bears potential as a new biodiesel feedstock. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 78 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 15 | 19% |
Student > Bachelor | 9 | 12% |
Researcher | 7 | 9% |
Professor | 7 | 9% |
Student > Ph. D. Student | 5 | 6% |
Other | 13 | 17% |
Unknown | 22 | 28% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 18 | 23% |
Biochemistry, Genetics and Molecular Biology | 12 | 15% |
Engineering | 5 | 6% |
Immunology and Microbiology | 4 | 5% |
Chemistry | 4 | 5% |
Other | 10 | 13% |
Unknown | 25 | 32% |