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Use of RNALater® Preservation for Virome Sequencing in Outbreak Settings

Overview of attention for article published in Frontiers in Microbiology, September 2017
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Title
Use of RNALater® Preservation for Virome Sequencing in Outbreak Settings
Published in
Frontiers in Microbiology, September 2017
DOI 10.3389/fmicb.2017.01888
Pubmed ID
Authors

Claudia Kohl, Merle Wegener, Andreas Nitsche, Andreas Kurth

Abstract

Outbreaks of infectious diseases may occur in animal and human populations; this underlines the need for suitable One Health approaches. During outbreak situations, straightforward identification of etiological agents is indispensable for taking countermeasures. A recently published protocol for metagenomic virus detection in clinical specimens (TUViD-VM) was developed for snap-frozen tissues which can be challenging to obtain. Here, we describe the use of RNALater(®) -treated tissue at ambient temperatures for virome sequencing. This study demonstrates that samples stored in RNALater(®) buffer yield similar results to those stored snap-frozen.

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The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 43 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 26%
Student > Master 5 12%
Student > Bachelor 4 9%
Student > Ph. D. Student 4 9%
Other 3 7%
Other 5 12%
Unknown 11 26%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 28%
Biochemistry, Genetics and Molecular Biology 6 14%
Environmental Science 3 7%
Medicine and Dentistry 3 7%
Veterinary Science and Veterinary Medicine 2 5%
Other 4 9%
Unknown 13 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 October 2017.
All research outputs
#14,594,271
of 24,885,505 outputs
Outputs from Frontiers in Microbiology
#11,103
of 28,434 outputs
Outputs of similar age
#163,197
of 325,687 outputs
Outputs of similar age from Frontiers in Microbiology
#277
of 508 outputs
Altmetric has tracked 24,885,505 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 28,434 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.5. This one has gotten more attention than average, scoring higher than 58% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 325,687 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 508 others from the same source and published within six weeks on either side of this one. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.