Title |
Aedes aegypti Molecular Responses to Zika Virus: Modulation of Infection by the Toll and Jak/Stat Immune Pathways and Virus Host Factors
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Published in |
Frontiers in Microbiology, October 2017
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DOI | 10.3389/fmicb.2017.02050 |
Pubmed ID | |
Authors |
Yesseinia I. Angleró-Rodríguez, Hannah J. MacLeod, Seokyoung Kang, Jenny S. Carlson, Natapong Jupatanakul, George Dimopoulos |
Abstract |
Zika (ZIKV) and dengue virus (DENV) are transmitted to humans by Aedes mosquitoes. However, the molecular interactions between the vector and ZIKV remain largely unexplored. In this work, we further investigated the tropism of ZIKV in two different Aedes aegypti strains and show that the virus infection kinetics, tissue migration, and susceptibility to infection differ between mosquito strains. We also compare the vector transcriptome changes upon ZIKV or DENV infection demonstrating that 40% of the mosquito's midgut infection-responsive transcriptome is virus-specific at 7 days after virus ingestion. Regulated genes included key factors of the mosquito's anti-viral immunity. Comparison of the ZIKV and DENV infection-responsive transcriptome data to those available for yellow fever virus and West Nile virus identified 26 genes likely to play key roles in virus infection of Aedes mosquitoes. Through reverse genetic analyses, we show that the Toll and the Jak/Stat innate immune pathways mediate increased resistance to ZIKV infection, and the conserved DENV host factors vATPase and inosine-5'-monophosphate dehydrogenase are also utilized for ZIKV infection. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 4 | 33% |
United Kingdom | 1 | 8% |
Unknown | 7 | 58% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 8 | 67% |
Scientists | 3 | 25% |
Practitioners (doctors, other healthcare professionals) | 1 | 8% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 170 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 30 | 18% |
Student > Master | 27 | 16% |
Student > Ph. D. Student | 25 | 15% |
Student > Bachelor | 21 | 12% |
Student > Doctoral Student | 15 | 9% |
Other | 18 | 11% |
Unknown | 34 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 44 | 26% |
Biochemistry, Genetics and Molecular Biology | 37 | 22% |
Immunology and Microbiology | 22 | 13% |
Medicine and Dentistry | 9 | 5% |
Social Sciences | 4 | 2% |
Other | 15 | 9% |
Unknown | 39 | 23% |