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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Genome Sequencing of Extended-Spectrum β-Lactamase (ESBL)-Producing Klebsiella pneumoniae Isolated from Pigs and Abattoir Workers in Cameroon
|
|---|---|
| Published in |
Frontiers in Microbiology, February 2018
|
| DOI | 10.3389/fmicb.2018.00188 |
| Pubmed ID | |
| Authors |
Luria L. Founou, Raspail C. Founou, Mushal Allam, Arshad Ismail, Cyrille F. Djoko, Sabiha Y. Essack |
| Abstract |
Background and objectives: Extended-spectrum β-lactamase (ESBL)-producingKlebsiella pneumoniaeis a serious public health issue globally. In this study, the antibiotic resistance genes, virulence factors, mobile genetic elements, and genetic lineages of circulating ESBL-producingK. pneumoniaestrains isolated from pigs and humans in Cameroonian abattoirs were investigated using whole genome sequencing (WGS), in order to ascertain zoonotic transmission (viz. from animals to humans and/or vice-versa) in the food chain.Methods:During March-October 2016, 288 nasal and rectal pooled samples from 432 pigs as well as nasal and hand swabs from 82 humans were collected from Cameroon and South Africa. Seven ESBL-producingK. pneumoniaecirculating in Cameroonian pig abattoirs were selected and their genomic DNA sequenced using an Illumina MiSeq platform. Generated reads werede novoassembled using the Qiagen CLC Genomics Workbench and SPAdes. The assembled contigs were annotated using RAST and antibiotic resistance genes, virulence factors, plasmids, and bacteriophages were identified with ResFinder, Virulence Finder, PlasmidFinder, and PHAST, respectively.Results:ESBL-producingK. pneumoniaewere detected in pigs (34/158; 21.52%) and exposed workers (8/71; 11.26%) in Cameroon only. The circulatingK. pneumoniaestrains were dominated principally by the sequence type (ST) 14 and 39. In addition, the "high-risk" ST307 clone and two novel STs assigned ST2958 and ST2959 were detected. Genomic analysis identified various antibiotic resistance genes associated with resistance to β-lactams, aminoglycosides, fluoroquinolones, macrolide, lincosamide and streptogramins, rifampicin, sulfonamides, trimethoprim, phenicols and tetracycline. None of the ESBL-producingK. pneumoniaeharbored virulence genes. IntermingledK. pneumoniaepopulations were observed between pig- and human-source within and across abattoirs in the country.Conclusion:Our study shows that ESBL-producingK. pneumoniaeis actively disseminating in pigs and occupationally exposed workers in Cameroonian pig abattoirs and is probably underestimated in the absence of molecular epidemiological studies. It suggests pigs, abattoir workers and food products as potential reservoirs and sources of zoonotic transmission in Cameroon. Our findings underline the existence of a potential unheeded food safety and public health threat associated with these resistant strains and reinforce the crucial importance of implementing appropriate food safety measures and promoting rational antibiotic use. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Cameroon | 1 | 17% |
| United States | 1 | 17% |
| France | 1 | 17% |
| Australia | 1 | 17% |
| Unknown | 2 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 6 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 125 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 125 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 21 | 17% |
| Student > Ph. D. Student | 20 | 16% |
| Researcher | 17 | 14% |
| Student > Postgraduate | 8 | 6% |
| Other | 7 | 6% |
| Other | 24 | 19% |
| Unknown | 28 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 19 | 15% |
| Immunology and Microbiology | 19 | 15% |
| Agricultural and Biological Sciences | 13 | 10% |
| Medicine and Dentistry | 11 | 9% |
| Veterinary Science and Veterinary Medicine | 4 | 3% |
| Other | 18 | 14% |
| Unknown | 41 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 September 2019.
All research outputs
#6,493,633
of 23,025,074 outputs
Outputs from Frontiers in Microbiology
#6,638
of 25,149 outputs
Outputs of similar age
#136,029
of 442,608 outputs
Outputs of similar age from Frontiers in Microbiology
#205
of 519 outputs
Altmetric has tracked 23,025,074 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 25,149 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,608 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 519 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.