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Attention Score in Context
| Title |
Genetic Analysis of Virulence Potential of Escherichia coli O104 Serotypes Isolated From Cattle Feces Using Whole Genome Sequencing
|
|---|---|
| Published in |
Frontiers in Microbiology, March 2018
|
| DOI | 10.3389/fmicb.2018.00341 |
| Pubmed ID | |
| Authors |
Pragathi B. Shridhar, Isha R. Patel, Jayanthi Gangiredla, Lance W. Noll, Xiaorong Shi, Jianfa Bai, Christopher A. Elkins, Nancy A. Strockbine, T. G. Nagaraja |
| Abstract |
Escherichia coli O104:H4, a Shiga toxin-producing hybrid pathotype that was implicated in a major foodborne outbreak in Germany in 2011, has not been detected in cattle. However, serotypes of O104, other than O104:H4, have been isolated from cattle feces, with O104:H7 being the most predominant. In this study, we investigated, based on whole genome sequence analyses, the virulence potential ofE. coliO104 strains isolated from cattle feces, since cattle are asymptomatic carriers ofE. coliO104. The genomes of ten bovineE. coliO104 strains (six O104:H7, one O104:H8, one O104:H12, and two O104:H23) and five O104:H7 isolated from human clinical cases were sequenced. Of all the bovine O104 serotypes (H7, H8, H12, and H23) that were included in the study, onlyE. coliO104:H7 serotype possessed Shiga toxins. Four of the six bovine O104:H7 strains and one of the five human strains carriedstx1c. Three human O104 strains carriedstx2, two were of subtype 2a, and one was 2d. Genomes ofstxcarrying bovine O104:H7 strains were larger than thestx-negative strains of O104:H7 or other serotypes. The genome sizes were proportional to the number of genes carried on the mobile genetic elements (phages, prophages, transposable elements and plasmids). Both bovine and human strains were negative for intimin and other genes associated with the type III secretory system and non-LEE encoded effectors. Plasmid-encoded virulence genes (ehxA, epeA, espP, katP) were also present in bovine and human strains. All O104 strains were negative for antimicrobial resistance genes, except one human strain. Phylogenetic analysis indicated that bovineE. coliO104 strains carrying the same flagellar antigen clustered together and STEC strains clustered separately from non-STEC strains. One of the human O104:H7 strains was phylogenetically closely related to and belonged to the same sequence type (ST-1817) as the bovine O104:H7 STEC strains. This suggests that the bovine feces could be a source of human illness caused byE. coliO104:H7 serotype. Because bovine O104:H7 strains carried virulence genes similar to human clinical strains and one of the human clinical strains was phylogenetically related to bovine strains, the serotype has the potential to be a diarrheagenic pathogen in humans. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 54 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 10 | 19% |
| Researcher | 8 | 15% |
| Student > Bachelor | 8 | 15% |
| Student > Ph. D. Student | 6 | 11% |
| Professor | 5 | 9% |
| Other | 11 | 20% |
| Unknown | 6 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 10 | 19% |
| Biochemistry, Genetics and Molecular Biology | 10 | 19% |
| Agricultural and Biological Sciences | 8 | 15% |
| Veterinary Science and Veterinary Medicine | 7 | 13% |
| Medicine and Dentistry | 3 | 6% |
| Other | 6 | 11% |
| Unknown | 10 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 March 2018.
All research outputs
#15,432,895
of 24,464,848 outputs
Outputs from Frontiers in Microbiology
#13,957
of 27,725 outputs
Outputs of similar age
#193,105
of 335,464 outputs
Outputs of similar age from Frontiers in Microbiology
#367
of 595 outputs
Altmetric has tracked 24,464,848 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 27,725 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 335,464 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 595 others from the same source and published within six weeks on either side of this one. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.