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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Expanding the Genetic Code of Lactococcus lactis and Escherichia coli to Incorporate Non-canonical Amino Acids for Production of Modified Lantibiotics
|
|---|---|
| Published in |
Frontiers in Microbiology, April 2018
|
| DOI | 10.3389/fmicb.2018.00657 |
| Pubmed ID | |
| Authors |
Maike Bartholomae, Tobias Baumann, Jessica H. Nickling, David Peterhoff, Ralf Wagner, Nediljko Budisa, Oscar P. Kuipers |
| Abstract |
The incorporation of non-canonical amino acids (ncAAs) into ribosomally synthesized and post-translationally modified peptides, e.g., nisin from the Gram-positive bacterium Lactococcus lactis, bears great potential to expand the chemical space of various antimicrobials. The ncAA Nε-Boc-L-lysine (BocK) was chosen for incorporation into nisin using the archaeal pyrrolysyl-tRNA synthetase-tRNAPyl pair to establish orthogonal translation in L. lactis for read-through of in-frame amber stop codons. In parallel, recombinant nisin production and orthogonal translation were combined in Escherichia coli cells. Both organisms synthesized bioactive nisin(BocK) variants. Screening of a nisin amber codon library revealed suitable sites for ncAA incorporation and two variants displayed high antimicrobial activity. Orthogonal translation in E. coli and L. lactis presents a promising tool to create new-to-nature nisin derivatives. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 2 | 50% |
| Ireland | 1 | 25% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 75% |
| Members of the public | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 47 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 23% |
| Student > Bachelor | 7 | 15% |
| Student > Ph. D. Student | 6 | 13% |
| Student > Doctoral Student | 4 | 9% |
| Other | 2 | 4% |
| Other | 7 | 15% |
| Unknown | 10 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 19 | 40% |
| Agricultural and Biological Sciences | 5 | 11% |
| Immunology and Microbiology | 4 | 9% |
| Chemistry | 3 | 6% |
| Chemical Engineering | 1 | 2% |
| Other | 3 | 6% |
| Unknown | 12 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 April 2018.
All research outputs
#13,662,605
of 23,577,761 outputs
Outputs from Frontiers in Microbiology
#10,309
of 26,068 outputs
Outputs of similar age
#167,252
of 330,867 outputs
Outputs of similar age from Frontiers in Microbiology
#295
of 593 outputs
Altmetric has tracked 23,577,761 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 26,068 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has gotten more attention than average, scoring higher than 58% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 330,867 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 593 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.