You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Title |
NADH-Mediated Gene Expression in Streptococcus pneumoniae and Role of Rex as a Transcriptional Repressor of the Rex-Regulon
|
|---|---|
| Published in |
Frontiers in Microbiology, June 2018
|
| DOI | 10.3389/fmicb.2018.01300 |
| Pubmed ID | |
| Authors |
Muhammad Afzal, Sulman Shafeeq, Oscar P. Kuipers |
| Abstract |
Nicotinamide adenine dinucleotides (NAD(H)) play a vital role in various biological processes, including keeping the cellular redox balance. In this study, we investigate the regulatory responses of Streptococcus pneumoniae D39 to NADH and characterize the role of the Rex protein as a transcriptional repressor of the gapN, fba, pncB, adhB2, gap, and adhE genes. Transcriptomic analysis was used to observe the response of S. pneumoniae D39 to NADH. Our microarray studies revealed elevated expression of various genes/operons involved in transport and biosynthesis of niacin (gapN, fba, pncB, adhB2, gap, and adhE). Promoter lacZ-fusion assays and microarray studies with the rex mutant revealed the role of Rex as a transcriptional repressor of gapN, fba, pncB, adhB2, gap, and adhE involved in niacin uptake and biosynthesis, in the presence of NADH. We predict the operator site (5'-TTGTKAWAAWWTTCACAA-3') of Rex in the regulatory regions of Rex-regulated genes that was subsequently validated by promoter mutational experiments. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 27% |
| Student > Bachelor | 2 | 18% |
| Student > Doctoral Student | 1 | 9% |
| Student > Master | 1 | 9% |
| Student > Postgraduate | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 3 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 3 | 27% |
| Biochemistry, Genetics and Molecular Biology | 2 | 18% |
| Agricultural and Biological Sciences | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Engineering | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 3 | 27% |