Title |
Bacterial Memory of Persisters: Bacterial Persister Cells Can Retain Their Phenotype for Days or Weeks After Withdrawal From Colony–Biofilm Culture
|
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Published in |
Frontiers in Microbiology, June 2018
|
DOI | 10.3389/fmicb.2018.01396 |
Pubmed ID | |
Authors |
Saki Miyaue, Erika Suzuki, Yoko Komiyama, Yu Kondo, Miki Morikawa, Sumio Maeda |
Abstract |
Persister cells, or persisters, are a specific subpopulation of bacterial cells that have acquired temporary antibiotic-resistant phenotypes. In this study, we showed that Escherichia coli produces many more persister cells in colony-biofilm culture than in the usual liquid culture and that these persisters can be maintained in higher numbers than those from liquid culture for up to 4 weeks at 37°C in a fresh, nutrient-rich, antibiotic-containing medium, even after complete withdrawal from the colony-biofilm culture. This suggests the presence of a long-retention effect, or "memory effect", in the persister cell state of E. coli cells. We also discovered that such increases in persisters during colony-biofilm culture and their memory effects are common, to a greater or lesser degree, in other bacterial species. This is true not only for gram-negative bacteria (Acinetobacter and Salmonella) but also for gram-positive bacteria (Staphylococcus and Bacillus). This is the first report to suggest the presence of a common memory mechanism for the persister cell state, which is inscribed during colony-biofilm culture, in a wide variety of bacteria. |
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Geographical breakdown
Country | Count | As % |
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United States | 3 | 15% |
United Kingdom | 3 | 15% |
Germany | 1 | 5% |
Sweden | 1 | 5% |
Australia | 1 | 5% |
France | 1 | 5% |
Chile | 1 | 5% |
Canada | 1 | 5% |
Netherlands | 1 | 5% |
Other | 0 | 0% |
Unknown | 7 | 35% |
Demographic breakdown
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Scientists | 13 | 65% |
Members of the public | 4 | 20% |
Science communicators (journalists, bloggers, editors) | 2 | 10% |
Practitioners (doctors, other healthcare professionals) | 1 | 5% |
Mendeley readers
Geographical breakdown
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Unknown | 179 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 36 | 20% |
Student > Bachelor | 26 | 15% |
Student > Master | 23 | 13% |
Researcher | 20 | 11% |
Student > Doctoral Student | 12 | 7% |
Other | 19 | 11% |
Unknown | 43 | 24% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 19 | 11% |
Veterinary Science and Veterinary Medicine | 7 | 4% |
Environmental Science | 5 | 3% |
Other | 27 | 15% |
Unknown | 55 | 31% |