Title |
Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates From Different Host Species
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Published in |
Frontiers in Microbiology, June 2018
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DOI | 10.3389/fmicb.2018.01408 |
Pubmed ID | |
Authors |
Zhong Peng, Wan Liang, Fei Wang, Zhuofei Xu, Zhihao Xie, Zhenghan Lian, Lin Hua, Rui Zhou, Huanchun Chen, Bin Wu |
Abstract |
Pasteurella multocida is a leading cause of respiratory diseases in many host species. To understand the genetic characteristics of P. multocida strains isolated from different host species, we sequenced the genomic DNA of P. multocida isolated from pigs and analyzed the genetic characteristics of strains from avian species, bovine species, pigs, and rabbits using whole genome sequence (WGS) data. Our results found that a capsular: lipopolysaccharide (LPS): multilocus sequence typing (MLST) genotype A: L1: ST129 (43.75%) was predominant in avian P. multocida; while genotypes B: L2: ST122 (60.00%) and A: L3: ST79 (30.00%) were predominate in bovine P. multocida; genotype D: L6: ST50 (37.50%) in porcine P. multocida; and genotype A: L3: ST9 (76.47%) in rabbit P. multocida. Comparative genomic analysis of P. multocida from different host species found that there are no genes in the P. multocida genome that are specific to any type of host. Phylogenetic analysis using either whole-genome single nucleotide polymorphisms (SNPs) or the set of SNPs present in all single-copy core genes across genomes showed that P. multocida strains with the same LPS genotype and MLST genotype were clustered together, suggesting the combining both the LPS and MLST typing schemes better explained the topology seen in the P. multocida phylogeny. |
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Canada | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 56 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 8 | 14% |
Researcher | 7 | 13% |
Student > Master | 6 | 11% |
Student > Bachelor | 4 | 7% |
Student > Postgraduate | 2 | 4% |
Other | 6 | 11% |
Unknown | 23 | 41% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 9 | 16% |
Veterinary Science and Veterinary Medicine | 8 | 14% |
Biochemistry, Genetics and Molecular Biology | 5 | 9% |
Immunology and Microbiology | 4 | 7% |
Medicine and Dentistry | 2 | 4% |
Other | 2 | 4% |
Unknown | 26 | 46% |