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Attention Score in Context
| Title |
Functional Expression and Characterization of Tetrachloroethene Dehalogenase From Geobacter sp.
|
|---|---|
| Published in |
Frontiers in Microbiology, August 2018
|
| DOI | 10.3389/fmicb.2018.01774 |
| Pubmed ID | |
| Authors |
Ryuki Nakamura, Tomohiro Obata, Ryota Nojima, Yohey Hashimoto, Keiichi Noguchi, Takahiro Ogawa, Masafumi Yohda |
| Abstract |
Reductive dehalogenase (RDase) consists of two parts, RdhA and RdhB. RdhA is the catalytic subunit, harboring a cobalamin cofactor and two Fe-S clusters. RdhA is anchored to the cytoplasmic membrane via the membrane anchoring subunit, RdhB. There are many genes encoding RDases in the genome of organohalide-respiring bacteria, including Dehalococcoides spp. However, most genes have not been functionally characterized. Biochemical studies on RDases have been hampered by difficulties encountered in their expression and purification. In this study, we have expressed, purified and characterized RdhA of RDase for tetrachloroethene (PceA) from Geobacter sp. PceA was expressed as a fusion protein with a trigger factor tag in Escherichia coli. PceA was purified and denatured in aerobic condition. Subsequently, this protein was refolded in the presence of FeCl3, Na2S and cobalamin in anaerobic condition. The reconstituted PceA exhibited dechlorination ability for tetrachloroethene. UV-Vis spectroscopy has shown that it contains cobalamin and Fe-S clusters. Since this method requires anaerobic manipulation only in the reconstituting process and has a relatively high yield, it will enable further biochemical studies of RDases. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 25% |
| Researcher | 6 | 21% |
| Student > Bachelor | 4 | 14% |
| Student > Master | 2 | 7% |
| Student > Postgraduate | 2 | 7% |
| Other | 1 | 4% |
| Unknown | 6 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 25% |
| Environmental Science | 4 | 14% |
| Agricultural and Biological Sciences | 3 | 11% |
| Chemical Engineering | 2 | 7% |
| Chemistry | 2 | 7% |
| Other | 1 | 4% |
| Unknown | 9 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 August 2018.
All research outputs
#18,647,094
of 23,100,534 outputs
Outputs from Frontiers in Microbiology
#19,675
of 25,279 outputs
Outputs of similar age
#254,705
of 331,118 outputs
Outputs of similar age from Frontiers in Microbiology
#573
of 749 outputs
Altmetric has tracked 23,100,534 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 25,279 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 331,118 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 749 others from the same source and published within six weeks on either side of this one. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.