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Characterization of a Novel Mitochondrial Ascorbate Transporter From Rat Liver and Potato Mitochondria

Overview of attention for article published in Frontiers in Molecular Biosciences, June 2018
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  • Above-average Attention Score compared to outputs of the same age (53rd percentile)
  • Good Attention Score compared to outputs of the same age and source (65th percentile)

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Title
Characterization of a Novel Mitochondrial Ascorbate Transporter From Rat Liver and Potato Mitochondria
Published in
Frontiers in Molecular Biosciences, June 2018
DOI 10.3389/fmolb.2018.00058
Pubmed ID
Authors

Vito Scalera, Nicola Giangregorio, Silvana De Leonardis, Lara Console, Emanuele Salvatore Carulli, Annamaria Tonazzi

Abstract

The Mitochondrial Ascorbic Acid Transporter (MAT) from both rat liver and potato mitochondria has been reconstituted in proteoliposomes. The protein has a molecular mass in the range of 28-35 kDa and catalyzes saturable, temperature and pH dependent, unidirectional ascorbic acid transport. The transport activity is sodium independent and it is optimal at acidic pH values. It is stimulated by proton gradient, thus supporting that ascorbate is symported with H+. It is efficiently inhibited by the lysine reagent pyridoxal phosphate and it is not affected by inhibitors of other recognized plasma and mitochondrial membranes ascorbate transporters GLUT1(glucose transporter-1) or SVCT2 (sodium-dependent vitamin C transporter-2). Rat protein catalyzes a cooperative ascorbate transport, being involved two binding sites; the measured K0.5 is 1.5 mM. Taking into account the experimental results we propose that the reconstituted ascorbate transporter is not a GLUT or SVCT, since it shows different biochemical features. Data of potato transporter overlap the mammalian ones, except for the kinetic parameters non-experimentally measurable, thus supporting the MAT in plants fulfills the same transport role.

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X Demographics

The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 20%
Student > Master 3 20%
Professor 2 13%
Student > Ph. D. Student 2 13%
Student > Bachelor 1 7%
Other 1 7%
Unknown 3 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 47%
Nursing and Health Professions 2 13%
Agricultural and Biological Sciences 1 7%
Social Sciences 1 7%
Medicine and Dentistry 1 7%
Other 0 0%
Unknown 3 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 November 2022.
All research outputs
#7,557,593
of 23,053,169 outputs
Outputs from Frontiers in Molecular Biosciences
#731
of 3,890 outputs
Outputs of similar age
#130,263
of 328,979 outputs
Outputs of similar age from Frontiers in Molecular Biosciences
#11
of 29 outputs
Altmetric has tracked 23,053,169 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,890 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 328,979 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.