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Mendeley readers
Attention Score in Context
| Title |
Single cell electroporation for longitudinal imaging of synaptic structure and function in the adult mouse neocortex in vivo
|
|---|---|
| Published in |
Frontiers in Neuroanatomy, April 2015
|
| DOI | 10.3389/fnana.2015.00036 |
| Pubmed ID | |
| Authors |
Stéphane Pagès, Michele Cane, Jérôme Randall, Luca Capello, Anthony Holtmaat |
| Abstract |
Longitudinal imaging studies of neuronal structures in vivo have revealed rich dynamics in dendritic spines and axonal boutons. Spines and boutons are considered to be proxies for synapses. This implies that synapses display similar dynamics. However, spines and boutons do not always bear synapses, some may contain more than one, and dendritic shaft synapses have no clear structural proxies. In addition, synaptic strength is not always accurately revealed by just the size of these structures. Structural and functional dynamics of synapses could be studied more reliably using fluorescent synaptic proteins as markers for size and function. These proteins are often large and possibly interfere with circuit development, which renders them less suitable for conventional transfection or transgenesis methods such as viral vectors, in utero electroporation, and germline transgenesis. Single cell electroporation (SCE) has been shown to be a potential alternative for transfection of recombinant fluorescent proteins in adult cortical neurons. Here we provide proof of principle for the use of SCE to express and subsequently image fluorescently tagged synaptic proteins over days to weeks in vivo. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Germany | 1 | 2% |
| Switzerland | 1 | 2% |
| Unknown | 62 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 23 | 35% |
| Student > Ph. D. Student | 22 | 34% |
| Professor | 4 | 6% |
| Student > Postgraduate | 4 | 6% |
| Student > Master | 3 | 5% |
| Other | 4 | 6% |
| Unknown | 5 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 31 | 48% |
| Agricultural and Biological Sciences | 20 | 31% |
| Medicine and Dentistry | 4 | 6% |
| Biochemistry, Genetics and Molecular Biology | 2 | 3% |
| Nursing and Health Professions | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 6 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 April 2015.
All research outputs
#15,333,503
of 22,805,349 outputs
Outputs from Frontiers in Neuroanatomy
#786
of 1,159 outputs
Outputs of similar age
#157,775
of 264,831 outputs
Outputs of similar age from Frontiers in Neuroanatomy
#21
of 33 outputs
Altmetric has tracked 22,805,349 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,159 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.0. This one is in the 26th percentile – i.e., 26% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,831 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.