You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
Cell-attached recordings of responses evoked by photorelease of GABA in the immature cortical neurons
|
|---|---|
| Published in |
Frontiers in Cellular Neuroscience, January 2013
|
| DOI | 10.3389/fncel.2013.00083 |
| Pubmed ID | |
| Authors |
Marat Minlebaev, Guzel Valeeva, Vadim Tcheremiskine, Gaëlle Coustillier, Rustem Khazipov |
| Abstract |
We present a novel non-invasive technique to measure the polarity of GABAergic responses based on cell-attached recordings of currents activated by laser-uncaging of GABA. For these recordings, a patch pipette was filled with a solution containing RuBi-GABA, and GABA was released from this complex by a laser beam conducted to the tip of the patch pipette via an optic fiber. In cell-attached recordings from neocortical and hippocampal neurons in postnatal days P2-5 rat brain slices in vitro, we found that laser-uncaging of GABA activates integral cell-attached currents mediated by tens of GABA(A) channels. The initial response was inwardly directed, indicating a depolarizing response to GABA. The direction of the initial response was dependent on the pipette potential and analysis of its slope-voltage relationships revealed a depolarizing driving force of +11 mV for the currents through GABA channels. Initial depolarizing responses to GABA uncaging were inverted to hyperpolarizing in the presence of the NKCC1 blocker bumetanide. Current-voltage relationships of the currents evoked by RuBi-GABA uncaging using voltage-ramps at the peak of responses not only revealed a bumetanide-sensitive depolarizing reversal potential of the GABA(A) receptor mediated responses, but also showed a strong voltage-dependent hysteresis. Upon desensitization of the uncaged-GABA response, current-voltage relationships of the currents through single GABA(A) channels revealed depolarizing responses with the driving force values similar to those obtained for the initial response. Thus, cell-attached recordings of the responses evoked by local intrapipette GABA uncaging are suitable to assess the polarity of the GABA(A)-Rs mediated signals in small cell compartments. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 50% |
| Japan | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 2 | 4% |
| Sweden | 1 | 2% |
| Germany | 1 | 2% |
| Finland | 1 | 2% |
| United States | 1 | 2% |
| Unknown | 42 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 21 | 44% |
| Student > Ph. D. Student | 10 | 21% |
| Student > Doctoral Student | 5 | 10% |
| Student > Bachelor | 3 | 6% |
| Professor | 2 | 4% |
| Other | 2 | 4% |
| Unknown | 5 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 18 | 38% |
| Agricultural and Biological Sciences | 14 | 29% |
| Engineering | 4 | 8% |
| Medicine and Dentistry | 3 | 6% |
| Physics and Astronomy | 2 | 4% |
| Other | 2 | 4% |
| Unknown | 5 | 10% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 June 2013.
All research outputs
#17,689,426
of 22,711,242 outputs
Outputs from Frontiers in Cellular Neuroscience
#2,909
of 4,210 outputs
Outputs of similar age
#210,175
of 280,736 outputs
Outputs of similar age from Frontiers in Cellular Neuroscience
#124
of 203 outputs
Altmetric has tracked 22,711,242 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,210 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,736 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 203 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.