Title |
Sonic Hedgehog modulates EGFR dependent proliferation of neural stem cells during late mouse embryogenesis through EGFR transactivation
|
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Published in |
Frontiers in Cellular Neuroscience, January 2013
|
DOI | 10.3389/fncel.2013.00166 |
Pubmed ID | |
Authors |
Gisela Reinchisi, Margarita Parada, Pablo Lois, Claudia Oyanadel, Ronan Shaughnessy, Alfonso Gonzalez, Verónica Palma |
Abstract |
Sonic Hedgehog (Shh/GLI) and EGFR signaling pathways modulate Neural Stem Cell (NSC) proliferation. How these signals cooperate is therefore critical for understanding normal brain development and function. Here we report a novel acute effect of Shh signaling on EGFR function. We show that during late neocortex development, Shh mediates the activation of the ERK1/2 signaling pathway in Radial Glial cells (RGC) through EGFR transactivation. This process is dependent on metalloprotease activity and accounts for almost 50% of the EGFR-dependent mitogenic response of late NSCs. Furthermore, in HeLa cancer cells, a well-known model for studying the EGFR receptor function, Shh also induces cell proliferation involving EGFR activation, as reflected by EGFR internalization and ERK1/2 phosphorylation. These findings may have important implications for understanding the mechanisms that regulate NSC proliferation during neurogenesis and may lead to novel approaches to the treatment of tumors. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Japan | 1 | 3% |
Czechia | 1 | 3% |
Canada | 1 | 3% |
Unknown | 31 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 13 | 38% |
Researcher | 6 | 18% |
Student > Bachelor | 3 | 9% |
Professor | 1 | 3% |
Student > Doctoral Student | 1 | 3% |
Other | 2 | 6% |
Unknown | 8 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 26% |
Agricultural and Biological Sciences | 9 | 26% |
Medicine and Dentistry | 3 | 9% |
Neuroscience | 2 | 6% |
Physics and Astronomy | 1 | 3% |
Other | 1 | 3% |
Unknown | 9 | 26% |