Title |
Identification of circulating microRNAs for the differential diagnosis of Parkinson's disease and Multiple System Atrophy
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Published in |
Frontiers in Cellular Neuroscience, June 2014
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DOI | 10.3389/fncel.2014.00156 |
Pubmed ID | |
Authors |
Annamaria Vallelunga, Marco Ragusa, Stefania Di Mauro, Tommaso Iannitti, Manuela Pilleri, Roberta Biundo, Luca Weis, Cinzia Di Pietro, Angela De Iuliis, Alessandra Nicoletti, Mario Zappia, Michele Purrello, Angelo Antonini |
Abstract |
Background: Parkinson's disease (PD) is a progressive neurodegenerative disorder which may be misdiagnosed with atypical conditions such as Multiple System Atrophy (MSA), due to overlapping clinical features. MicroRNAs (miRNAs) are small non-coding RNAs with a key role in post-transcriptional gene regulation. We hypothesized that identification of a distinct set of circulating miRNAs (cmiRNAs) could distinguish patients affected by PD from MSA and healthy individuals. Results. Using TaqMan Low Density Array technology, we analyzed 754 miRNAs and found 9 cmiRNAs differentially expressed in PD and MSA patients compared to healthy controls. We also validated a set of 4 differentially expressed cmiRNAs in PD and MSA patients vs. controls. More specifically, miR-339-5p was downregulated, whereas miR-223(*), miR-324-3p, and mir-24 were upregulated in both diseases. We found cmiRNAs specifically deregulated in PD (downregulation of miR-30c and miR-148b) and in MSA (upregulation of miR-148b). Finally, comparing MSA and PD, we identified 3 upregulated cmiRNAs in MSA serum (miR-24, miR-34b, miR-148b). Conclusions. Our results suggest that cmiRNA signatures discriminate PD from MSA patients and healthy controls and may be considered specific, non-invasive biomarkers for differential diagnosis. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Italy | 2 | 50% |
Unknown | 2 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 75% |
Practitioners (doctors, other healthcare professionals) | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | <1% |
Italy | 1 | <1% |
India | 1 | <1% |
Czechia | 1 | <1% |
Japan | 1 | <1% |
United States | 1 | <1% |
Unknown | 145 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 29 | 19% |
Student > Ph. D. Student | 25 | 17% |
Student > Master | 15 | 10% |
Student > Doctoral Student | 15 | 10% |
Student > Bachelor | 13 | 9% |
Other | 27 | 18% |
Unknown | 27 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 27 | 18% |
Neuroscience | 25 | 17% |
Agricultural and Biological Sciences | 25 | 17% |
Biochemistry, Genetics and Molecular Biology | 15 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 6 | 4% |
Other | 16 | 11% |
Unknown | 37 | 25% |