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Attention Score in Context
| Title |
Bruchpilot and Synaptotagmin collaborate to drive rapid glutamate release and active zone differentiation
|
|---|---|
| Published in |
Frontiers in Cellular Neuroscience, February 2015
|
| DOI | 10.3389/fncel.2015.00029 |
| Pubmed ID | |
| Authors |
Mila M. Paul, Martin Pauli, Nadine Ehmann, Stefan Hallermann, Markus Sauer, Robert J. Kittel, Manfred Heckmann |
| Abstract |
The active zone (AZ) protein Bruchpilot (Brp) is essential for rapid glutamate release at Drosophila melanogaster neuromuscular junctions (NMJs). Quantal time course and measurements of action potential-waveform suggest that presynaptic fusion mechanisms are altered in brp null mutants (brp(69) ). This could account for their increased evoked excitatory postsynaptic current (EPSC) delay and rise time (by about 1 ms). To test the mechanism of release protraction at brp(69) AZs, we performed knock-down of Synaptotagmin-1 (Syt) via RNAi (syt(KD) ) in wildtype (wt), brp(69) and rab3 null mutants (rab3(rup) ), where Brp is concentrated at a small number of AZs. At wt and rab3(rup) synapses, syt(KD) lowered EPSC amplitude while increasing rise time and delay, consistent with the role of Syt as a release sensor. In contrast, syt(KD) did not alter EPSC amplitude at brp(69) synapses, but shortened delay and rise time. In fact, following syt(KD) , these kinetic properties were strikingly similar in wt and brp(69) , which supports the notion that Syt protracts release at brp(69) synapses. To gain insight into this surprising role of Syt at brp(69) AZs, we analyzed the structural and functional differentiation of synaptic boutons at the NMJ. At 'tonic' type Ib motor neurons, distal boutons contain more AZs, more Brp proteins per AZ and show elevated and accelerated glutamate release compared to proximal boutons. The functional differentiation between proximal and distal boutons is Brp-dependent and reduced after syt(KD) . Notably, syt(KD) boutons are smaller, contain fewer Brp positive AZs and these are of similar number in proximal and distal boutons. In addition, super-resolution imaging via dSTORM revealed that syt(KD) increases the number and alters the spatial distribution of Brp molecules at AZs, while the gradient of Brp proteins per AZ is diminished. In summary, these data demonstrate that normal structural and functional differentiation of Drosophila AZs requires concerted action of Brp and Syt. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 5% |
| Unknown | 61 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 24 | 38% |
| Student > Doctoral Student | 13 | 20% |
| Researcher | 10 | 16% |
| Student > Bachelor | 6 | 9% |
| Professor | 2 | 3% |
| Other | 6 | 9% |
| Unknown | 3 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 22 | 34% |
| Agricultural and Biological Sciences | 19 | 30% |
| Biochemistry, Genetics and Molecular Biology | 8 | 13% |
| Medicine and Dentistry | 6 | 9% |
| Immunology and Microbiology | 1 | 2% |
| Other | 4 | 6% |
| Unknown | 4 | 6% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 February 2015.
All research outputs
#15,330,390
of 23,577,654 outputs
Outputs from Frontiers in Cellular Neuroscience
#2,487
of 4,388 outputs
Outputs of similar age
#202,672
of 355,680 outputs
Outputs of similar age from Frontiers in Cellular Neuroscience
#45
of 90 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,388 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
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We're also able to compare this research output to 90 others from the same source and published within six weeks on either side of this one. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.