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X Demographics
Mendeley readers
Attention Score in Context
| Title |
A Method for the Isolation and Culture of Adult Rat Retinal Pigment Epithelial (RPE) Cells to Study Retinal Diseases
|
|---|---|
| Published in |
Frontiers in Cellular Neuroscience, November 2015
|
| DOI | 10.3389/fncel.2015.00449 |
| Pubmed ID | |
| Authors |
Janosch P. Heller, Jessica C. F. Kwok, Elena Vecino, Keith R. Martin, James W. Fawcett |
| Abstract |
Diseases such as age-related macular degeneration (AMD) affect the retinal pigment epithelium (RPE) and lead to the death of the epithelial cells and ultimately blindness. RPE transplantation is currently a major focus of eye research and clinical trials using human stem cell-derived RPE cells are ongoing. However, it remains to be established to which extent the source of RPE cells for transplantation affects their therapeutic efficacy and this needs to be explored in animal models. Autotransplantation of RPE cells has attractions as a therapy, but existing protocols to isolate adult RPE cells from rodents are technically difficult, time-consuming, have a low yield and are not optimized for long-term cell culturing. Here, we report a newly devised protocol which facilitates reliable and simple isolation and culture of RPE cells from adult rats. Incubation of a whole rat eyeball in 20 U/ml papain solution for 50 min yielded 4 × 10(4) viable RPE cells. These cells were hexagonal and pigmented upon culture. Using immunostaining, we demonstrated that the cells expressed RPE cell-specific marker proteins including cytokeratin 18 and RPE65, similar to RPE cells in vivo. Additionally, the cells were able to produce and secrete Bruch's membrane matrix components similar to in vivo situation. Similarly, the cultured RPE cells adhered to isolated Bruch's membrane as has previously been reported. Therefore, the protocol described in this article provides an efficient method for the rapid and easy isolation of high quantities of adult rat RPE cells. This provides a reliable platform for studying the therapeutic targets, testing the effects of drugs in a preclinical setup and to perform in vitro and in vivo transplantation experiments to study retinal diseases. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 33% |
| Switzerland | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 77 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Iran, Islamic Republic of | 1 | 1% |
| Australia | 1 | 1% |
| Unknown | 75 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 17 | 22% |
| Student > Ph. D. Student | 11 | 14% |
| Student > Master | 10 | 13% |
| Student > Bachelor | 10 | 13% |
| Student > Doctoral Student | 3 | 4% |
| Other | 12 | 16% |
| Unknown | 14 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 14 | 18% |
| Agricultural and Biological Sciences | 14 | 18% |
| Medicine and Dentistry | 12 | 16% |
| Biochemistry, Genetics and Molecular Biology | 8 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 6% |
| Other | 6 | 8% |
| Unknown | 18 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 January 2016.
All research outputs
#14,828,686
of 22,833,393 outputs
Outputs from Frontiers in Cellular Neuroscience
#2,396
of 4,248 outputs
Outputs of similar age
#214,473
of 386,526 outputs
Outputs of similar age from Frontiers in Cellular Neuroscience
#59
of 109 outputs
Altmetric has tracked 22,833,393 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,248 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 386,526 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 109 others from the same source and published within six weeks on either side of this one. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.