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Mendeley readers
Attention Score in Context
| Title |
An Optimized Approach to Recover Secreted Proteins from Fibroblast Conditioned-Media for Secretomic Analysis
|
|---|---|
| Published in |
Frontiers in Cellular Neuroscience, March 2016
|
| DOI | 10.3389/fncel.2016.00070 |
| Pubmed ID | |
| Authors |
Bastien Paré, Lydia T. Deschênes, Roxane Pouliot, Nicolas Dupré, Francois Gros-Louis |
| Abstract |
The proteins secreted by a particular type of cell, the secretome, play important roles in the regulation of many physiological processes via paracrine/autocrine mechanisms, and they are of increasing interest to help understanding rare diseases and to identify potential biomarkers and therapeutic targets. To facilitate ongoing research involving secreted proteins, we revisited cell culture protocols and whole secreted protein enrichment protocols. A reliable method for culturing and precipitating secreted protein from patient-derived fibroblast conditioned-medium was established. The method is based on the optimization of cell confluency and incubation time conditions. The well-established carrier-based TCA-DOC protein precipitation method was consistently found to give higher protein recovery yield. According to our results, we therefore propose that protein enrichment should be performed by TCA-DOC precipitation method after 48 h at 95% of confluence in a serum-deprived culture medium. Given the importance of secreted proteins as a source to elucidate the pathogenesis of rare diseases, especially neurological disorders, this approach may help to discover novel candidate biomarkers with potential clinical significance. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 94 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 1% |
| Unknown | 93 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 22 | 23% |
| Student > Bachelor | 14 | 15% |
| Student > Master | 11 | 12% |
| Researcher | 9 | 10% |
| Student > Doctoral Student | 7 | 7% |
| Other | 9 | 10% |
| Unknown | 22 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 32 | 34% |
| Agricultural and Biological Sciences | 12 | 13% |
| Engineering | 8 | 9% |
| Medicine and Dentistry | 7 | 7% |
| Neuroscience | 4 | 4% |
| Other | 7 | 7% |
| Unknown | 24 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2016.
All research outputs
#20,317,110
of 22,858,915 outputs
Outputs from Frontiers in Cellular Neuroscience
#3,585
of 4,254 outputs
Outputs of similar age
#255,093
of 301,001 outputs
Outputs of similar age from Frontiers in Cellular Neuroscience
#77
of 93 outputs
Altmetric has tracked 22,858,915 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,254 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 93 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.