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X Demographics
Mendeley readers
Attention Score in Context
| Title |
A Novel Approach for Amplification and Purification of Mouse Oligodendrocyte Progenitor Cells
|
|---|---|
| Published in |
Frontiers in Cellular Neuroscience, August 2016
|
| DOI | 10.3389/fncel.2016.00203 |
| Pubmed ID | |
| Authors |
Junlin Yang, Xuejun Cheng, Jiaxi Shen, Binghua Xie, Xiaofeng Zhao, Zunyi Zhang, Qilin Cao, Ying Shen, Mengsheng Qiu |
| Abstract |
Although transgenic and knockout mice are widely used to study the specification and differentiation of oligodendrocyte precursor cells (OPCs), mouse primary OPCs are difficult to be purified and maintained, and many in vitro studies have to resort to rat OPCs as substitutes. In this study, we reported that mouse O4 negative early-stage OPCs can be obtained by culturing cortical tissue blocks, and the simultaneous treatment of OPCs with Platelet Derived Growth Factor-AA (PDGFaa), basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF) is the key for the propagation of mouse OPCs in culture. EGF was found to be a potent mitogen for OPCs and cooperate with PDGFaa to extend cell division and inhibit their differentiation. EGF also collaborates with PDGFaa and bFGF to convert bipolar or tripolar OPCs to more vital fibroblast-like OPCs without compromising their oligodendrocyte differentiation potential. In addition, EGF promoted the survival and proliferation of glial progenitor cells (GPCs) derived from primary OPC cultures, and a mixture of GPCs and OPCs can be obtained and propagated in the presence of EGF, bFGF, and PDGFaa. Once EGF is withdrawn, GPC population decreased sharply and fibroblast-like OPCs changed into typical OPCs morphology, then homogeneous OPCs were obtained subsequently. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 51 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 9 | 18% |
| Student > Ph. D. Student | 8 | 16% |
| Researcher | 7 | 14% |
| Student > Bachelor | 6 | 12% |
| Professor > Associate Professor | 3 | 6% |
| Other | 3 | 6% |
| Unknown | 15 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 13 | 25% |
| Agricultural and Biological Sciences | 10 | 20% |
| Biochemistry, Genetics and Molecular Biology | 5 | 10% |
| Immunology and Microbiology | 3 | 6% |
| Arts and Humanities | 1 | 2% |
| Other | 4 | 8% |
| Unknown | 15 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 September 2016.
All research outputs
#17,812,737
of 22,883,326 outputs
Outputs from Frontiers in Cellular Neuroscience
#2,946
of 4,256 outputs
Outputs of similar age
#248,840
of 343,744 outputs
Outputs of similar age from Frontiers in Cellular Neuroscience
#24
of 50 outputs
Altmetric has tracked 22,883,326 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,256 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 343,744 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 50 others from the same source and published within six weeks on either side of this one. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.