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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Tagging of Endogenous BK Channels with a Fluorogen-Activating Peptide Reveals β4-Mediated Control of Channel Clustering in Cerebellum
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|---|---|
| Published in |
Frontiers in Cellular Neuroscience, October 2017
|
| DOI | 10.3389/fncel.2017.00337 |
| Pubmed ID | |
| Authors |
Christopher P. Pratt, Dika A. Kuljis, Gregg E. Homanics, Jianjun He, Dmytro Kolodieznyi, Srikanth Dudem, Mark A. Hollywood, Alison L. Barth, Marcel P. Bruchez |
| Abstract |
BK channels are critical regulators of neuronal activity, controlling firing, neurotransmitter release, cerebellar function, and BK channel mutations have been linked to seizure disorders. Modulation of BK channel gating is well characterized, regulated by accessory subunit interactions, intracellular signaling pathways, and membrane potential. In contrast, the role of intracellular trafficking mechanisms in controlling BK channel function, especially in live cells, has been less studied. Fluorogen-activating peptides (FAPs) are well-suited for trafficking and physiological studies due to the binding of malachite green (MG)-based dyes with sub-nanomolar affinity to the FAP, resulting in bright, photostable, far-red fluorescence. Cell-excluded MG dyes enable the selective tagging of surface protein and tracking through endocytic pathways. We used CRISPR to insert the FAP at the extracellular N-terminus of BKα in the first exon of its native locus, enabling regulation by the native promoter elements and tag incorporation into multiple splice isoforms. Motor coordination was found to be normal; however, BK channel expression seems to be reduced in some locations. Alternate start site selection or post-translational proteolytic processing resulted in incomplete FAP tagging of the BKα proteins in brain tissues. In Purkinje cell somata, FAP revealed BK channel clustering previously only observed by electron microscopy. Measurement of these clusters in β4(+/-) and β4(-/-) mice showed that puncta number and cluster fluorescence intensity on the soma are reduced in β4(-/-) knockout animals. This novel mouse line provides a versatile fluorescent platform for studying endogenous BK channels in living and fixed tissues. Future studies could apply this line to ex vivo neuronal cultures to study live-cell channel trafficking. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 24% |
| Student > Bachelor | 5 | 20% |
| Researcher | 3 | 12% |
| Other | 1 | 4% |
| Professor | 1 | 4% |
| Other | 4 | 16% |
| Unknown | 5 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 20% |
| Neuroscience | 5 | 20% |
| Biochemistry, Genetics and Molecular Biology | 2 | 8% |
| Arts and Humanities | 2 | 8% |
| Engineering | 2 | 8% |
| Other | 3 | 12% |
| Unknown | 6 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 October 2017.
All research outputs
#15,330,390
of 23,577,654 outputs
Outputs from Frontiers in Cellular Neuroscience
#2,487
of 4,388 outputs
Outputs of similar age
#196,456
of 330,320 outputs
Outputs of similar age from Frontiers in Cellular Neuroscience
#64
of 116 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,388 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 330,320 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 116 others from the same source and published within six weeks on either side of this one. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.