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Mendeley readers
Attention Score in Context
| Title |
A Method to Culture GABAergic Interneurons Derived from the Medial Ganglionic Eminence
|
|---|---|
| Published in |
Frontiers in Cellular Neuroscience, January 2018
|
| DOI | 10.3389/fncel.2017.00423 |
| Pubmed ID | |
| Authors |
Sira A. Franchi, Romina Macco, Veronica Astro, Diletta Tonoli, Elisa Savino, Flavia Valtorta, Kristyna Sala, Martina Botta, Ivan de Curtis |
| Abstract |
Understanding the mechanisms guiding interneuron development is a central aspect of the current research on cortical/hippocampal interneurons, which is highly relevant to brain function and pathology. In this methodological study we have addressed the setup of protocols for the reproducible culture of dissociated cells from murine medial ganglionic eminences (MGEs), to provide a culture system for the analysis of interneurons in vitro. This study includes the detailed protocols for the preparation of the dissociated cells, and for their culture on optimal substrates for cell migration or differentiation. These cultures enriched in interneurons may allow the investigation of the migratory behavior of interneuron precursors and their differentiation in vitro, up to the formation of morphologically identifiable GABAergic synapses. Live imaging of MGE-derived cells plated on proper substrates shows that they are useful to study the migratory behavior of the precursors, as well as the behavior of growth cones during the development of neurites. Most MGE-derived precursors develop into polarized GABAergic interneurons as determined by axonal, dendritic, and GABAergic markers. We present also a comparison of cells from WT and mutant mice as a proof of principle for the use of these cultures for the analysis of the migration and differentiation of GABAergic cells with different genetic backgrounds. The culture enriched in interneurons described here represents a useful experimental system to examine in a relatively easy and fast way the morpho-functional properties of these cells under physiological or pathological conditions, providing a powerful tool to complement the studies in vivo. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| France | 1 | 33% |
| Switzerland | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 70 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 70 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 24% |
| Researcher | 13 | 19% |
| Student > Master | 9 | 13% |
| Student > Bachelor | 6 | 9% |
| Student > Doctoral Student | 4 | 6% |
| Other | 5 | 7% |
| Unknown | 16 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 27 | 39% |
| Agricultural and Biological Sciences | 10 | 14% |
| Biochemistry, Genetics and Molecular Biology | 10 | 14% |
| Medicine and Dentistry | 2 | 3% |
| Nursing and Health Professions | 1 | 1% |
| Other | 4 | 6% |
| Unknown | 16 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 July 2018.
All research outputs
#15,487,739
of 23,015,156 outputs
Outputs from Frontiers in Cellular Neuroscience
#2,686
of 4,264 outputs
Outputs of similar age
#270,239
of 442,237 outputs
Outputs of similar age from Frontiers in Cellular Neuroscience
#58
of 105 outputs
Altmetric has tracked 23,015,156 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,264 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,237 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 105 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.