Title |
Pretreatment with Sodium Phenylbutyrate Alleviates Cerebral Ischemia/Reperfusion Injury by Upregulating DJ-1 Protein
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Published in |
Frontiers in Neurology, June 2017
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DOI | 10.3389/fneur.2017.00256 |
Pubmed ID | |
Authors |
Rui-Xin Yang, Jie Lei, Bo-Dong Wang, Da-Yun Feng, Lu Huang, Yu-Qian Li, Tao Li, Gang Zhu, Chen Li, Fang-Fang Lu, Tie-Jian Nie, Guo-Dong Gao, Li Gao |
Abstract |
Oxidative stress and mitochondrial dysfunction play critical roles in ischemia/reperfusion (I/R) injury. DJ-1 is an endogenous antioxidant that attenuates oxidative stress and maintains mitochondrial function, likely acting as a protector of I/R injury. In the present study, we explored the protective effect of a possible DJ-1 agonist, sodium phenylbutyrate (SPB), against I/R injury by protecting mitochondrial dysfunction via the upregulation of DJ-1 protein. Pretreatment with SPB upregulated the DJ-1 protein level and rescued the I/R injury-induced DJ-1 decrease about 50% both in vivo and in vitro. SPB also improved cellular viability and mitochondrial function and alleviated neuronal apoptosis both in cell and animal models; these effects of SPB were abolished by DJ-1 knockdown with siRNA. Furthermore, SPB improved the survival rate about 20% and neurological functions, as well as reduced about 50% of the infarct volume and brain edema, of middle cerebral artery occlusion mice 23 h after reperfusion. Therefore, our findings demonstrate that preconditioning of SPB possesses a neuroprotective effect against cerebral I/R injury by protecting mitochondrial function dependent on the DJ-1 upregulation, suggesting that DJ-1 is a potential therapeutic target for clinical ischemic stroke. |
X Demographics
Geographical breakdown
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Switzerland | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 2 | 17% |
Student > Ph. D. Student | 2 | 17% |
Lecturer > Senior Lecturer | 1 | 8% |
Student > Master | 1 | 8% |
Researcher | 1 | 8% |
Other | 1 | 8% |
Unknown | 4 | 33% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 5 | 42% |
Neuroscience | 2 | 17% |
Unknown | 5 | 42% |