Title |
moco: Fast Motion Correction for Calcium Imaging
|
---|---|
Published in |
Frontiers in Neuroinformatics, February 2016
|
DOI | 10.3389/fninf.2016.00006 |
Pubmed ID | |
Authors |
Alexander Dubbs, James Guevara, Rafael Yuste |
Abstract |
Motion correction is the first step in a pipeline of algorithms to analyze calcium imaging videos and extract biologically relevant information, for example the network structure of the neurons therein. Fast motion correction is especially critical for closed-loop activity triggered stimulation experiments, where accurate detection and targeting of specific cells in necessary. We introduce a novel motion-correction algorithm which uses a Fourier-transform approach, and a combination of judicious downsampling and the accelerated computation of many L 2 norms using dynamic programming and two-dimensional, fft-accelerated convolutions, to enhance its efficiency. Its accuracy is comparable to that of established community-used algorithms, and it is more stable to large translational motions. It is programmed in Java and is compatible with ImageJ. |
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Mexico | 1 | 7% |
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Sweden | 1 | 7% |
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Switzerland | 1 | 7% |
Germany | 1 | 7% |
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Unknown | 5 | 33% |
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Scientists | 1 | 7% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 4 | 2% |
Japan | 2 | 1% |
France | 1 | <1% |
Unknown | 165 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 58 | 34% |
Researcher | 34 | 20% |
Student > Master | 18 | 10% |
Student > Postgraduate | 9 | 5% |
Student > Bachelor | 7 | 4% |
Other | 19 | 11% |
Unknown | 27 | 16% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 42 | 24% |
Engineering | 12 | 7% |
Physics and Astronomy | 8 | 5% |
Medicine and Dentistry | 8 | 5% |
Other | 16 | 9% |
Unknown | 34 | 20% |