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Initial photophysical characterization of the proteorhodopsin optical proton sensor (PROPS)

Overview of attention for article published in Frontiers in Neuroscience, September 2015
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Title
Initial photophysical characterization of the proteorhodopsin optical proton sensor (PROPS)
Published in
Frontiers in Neuroscience, September 2015
DOI 10.3389/fnins.2015.00315
Pubmed ID
Authors

Jay L. Nadeau

Abstract

Fluorescence is not frequently used as a tool for investigating the photocycles of rhodopsins, largely because of the low quantum yield of the retinal chromophore. However, a new class of genetically encoded voltage sensors is based upon rhodopsins and their fluorescence. The first such sensor reported in the literature was the proteorhodopsin optical proton sensor (PROPS), which is capable of indicating membrane voltage changes in bacteria by means of changes in fluorescence. However, the properties of this fluorescence, such as its lifetime decay components and its origin in the protein photocycle, remain unknown. This paper reports steady-state and nanosecond time-resolved emission of this protein expressed in two strains of Escherichia coli, before and after membrane depolarization. The voltage-dependence of a particularly long lifetime component is established. Additional work to improve quantum yields and improve the general utility of PROPS is suggested.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 3%
Unknown 31 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 22%
Student > Master 5 16%
Researcher 5 16%
Student > Bachelor 4 13%
Lecturer 2 6%
Other 5 16%
Unknown 4 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 28%
Biochemistry, Genetics and Molecular Biology 5 16%
Chemistry 5 16%
Physics and Astronomy 4 13%
Neuroscience 2 6%
Other 4 13%
Unknown 3 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 September 2022.
All research outputs
#19,942,887
of 25,371,288 outputs
Outputs from Frontiers in Neuroscience
#8,668
of 11,538 outputs
Outputs of similar age
#190,212
of 277,639 outputs
Outputs of similar age from Frontiers in Neuroscience
#94
of 128 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
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