A novel versatile hybrid infusion-multielectrode recording (HIME) system for acute drug delivery and multisite acquisition of neuronal activity in freely moving mice

Oleg Senkov, Andrey Mironov, Alexander Dityatev

To characterize information transfer in defined brain circuits involving multiple brain regions and to evaluate underlying molecular mechanisms and their dysregulation in major brain diseases, a simple and reliable system is ultimately required for electrophysiological recording of local field potentials (LFPs, or local EEG) in combination with local delivery of drugs, enzymes and gene expression-controlling viruses near the place of recording. Here we provide a new design of a versatile reusable hybrid infusion-recording (HIME) system which can be utilized in freely moving mice performing cognitive tasks. The HIME system allows monitoring neuronal activity in multiple layers in several brain structures. Here, we provide examples of bilateral injection and recordings of full spectrum of learning and memory related oscillations, i.e., theta (4-12 Hz), gamma (40-100) and ripple activity (130-150 Hz), in five hippocampal layers as well as in the CA1 and CA2 regions. Furthermore, the system is designed to be used for parallel recordings in the amygdala, cortex and other brain areas, before and after infusion of reagents of interest, either in or off a cognitive test. We anticipate that the HIME system can be particularly convenient to advance functional neuroglycobiological studies and molecular deciphering of mechanisms governing long-term memory consolidation.