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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Concurrent Imaging of Synaptic Vesicle Recycling and Calcium Dynamics
|
|---|---|
| Published in |
Frontiers in Molecular Neuroscience, January 2011
|
| DOI | 10.3389/fnmol.2011.00034 |
| Pubmed ID | |
| Authors |
Haiyan Li, Sarah M. Foss, Yuriy L. Dobryy, C. Kevin Park, Samuel Andrew Hires, Nathan C. Shaner, Roger Y. Tsien, Leslie C. Osborne, Susan M. Voglmaier |
| Abstract |
Synaptic transmission involves the calcium dependent release of neurotransmitter from synaptic vesicles. Genetically encoded optical probes emitting different wavelengths of fluorescent light in response to neuronal activity offer a powerful approach to understand the spatial and temporal relationship of calcium dynamics to the release of neurotransmitter in defined neuronal populations. To simultaneously image synaptic vesicle recycling and changes in cytosolic calcium, we developed a red-shifted reporter of vesicle recycling based on a vesicular glutamate transporter, VGLUT1-mOrange2 (VGLUT1-mOr2), and a presynaptically localized green calcium indicator, synaptophysin-GCaMP3 (SyGCaMP3) with a large dynamic range. The fluorescence of VGLUT1-mOr2 is quenched by the low pH of synaptic vesicles. Exocytosis upon electrical stimulation exposes the luminal mOr2 to the neutral extracellular pH and relieves fluorescence quenching. Reacidification of the vesicle upon endocytosis again reduces fluorescence intensity. Changes in fluorescence intensity thus monitor synaptic vesicle exo- and endocytosis, as demonstrated previously for the green VGLUT1-pHluorin. To monitor changes in calcium, we fused the synaptic vesicle protein synaptophysin to the recently improved calcium indicator GCaMP3. SyGCaMP3 is targeted to presynaptic varicosities, and exhibits changes in fluorescence in response to electrical stimulation consistent with changes in calcium concentration. Using real time imaging of both reporters expressed in the same synapses, we determine the time course of changes in VGLUT1 recycling in relation to changes in presynaptic calcium concentration. Inhibition of P/Q- and N-type calcium channels reduces calcium levels, as well as the rate of synaptic vesicle exocytosis and the fraction of vesicles released. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 175 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 1% |
| United States | 2 | 1% |
| Netherlands | 1 | <1% |
| France | 1 | <1% |
| Germany | 1 | <1% |
| Finland | 1 | <1% |
| Portugal | 1 | <1% |
| Japan | 1 | <1% |
| Kazakhstan | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 164 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 50 | 29% |
| Researcher | 42 | 24% |
| Professor > Associate Professor | 17 | 10% |
| Student > Bachelor | 15 | 9% |
| Professor | 8 | 5% |
| Other | 26 | 15% |
| Unknown | 17 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 84 | 48% |
| Neuroscience | 35 | 20% |
| Medicine and Dentistry | 13 | 7% |
| Biochemistry, Genetics and Molecular Biology | 12 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 2% |
| Other | 9 | 5% |
| Unknown | 18 | 10% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 June 2012.
All research outputs
#18,313,878
of 22,675,759 outputs
Outputs from Frontiers in Molecular Neuroscience
#2,240
of 2,826 outputs
Outputs of similar age
#159,968
of 180,328 outputs
Outputs of similar age from Frontiers in Molecular Neuroscience
#18
of 27 outputs
Altmetric has tracked 22,675,759 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,826 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 13th percentile – i.e., 13% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 180,328 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.