Title |
Fatty acids increase neuronal hypertrophy of Pten knockdown neurons
|
---|---|
Published in |
Frontiers in Molecular Neuroscience, April 2014
|
DOI | 10.3389/fnmol.2014.00030 |
Pubmed ID | |
Authors |
Catherine J. Fricano, Tyrone DeSpenza, Paul W. Frazel, Meijie Li, A. James O'Malley, Gary L. Westbrook, Bryan W. Luikart |
Abstract |
Phosphatase and tensin homolog (Pten) catalyzes the reverse reaction of PI3K by dephosphorylating PIP3 to PIP2. This negatively regulates downstream Akt/mTOR/S6 signaling resulting in decreased cellular growth and proliferation. Co-injection of a lentivirus knocking Pten down with a control lentivirus allows us to compare the effects of Pten knockdown between individual neurons within the same animal. We find that knockdown of Pten results in neuronal hypertrophy by 21 days post-injection. This neuronal hypertrophy is correlated with increased p-S6 and p-mTOR in individual neurons. We used this system to test whether an environmental factor that has been implicated in cellular hypertrophy could influence the severity of the Pten knockdown-induced hypertrophy. Implantation of mini-osmotic pumps delivering fatty acids results in increased neuronal hypertrophy and p-S6/p-mTOR staining. These hypertrophic effects were reversed in response to rapamycin treatment. However, we did not observe a similar increase in hypertrophy in response to dietary manipulations of fatty acids. Thus, we conclude that by driving growth signaling with fatty acids and knocking down a critical regulator of growth, Pten, we are able to observe an additive morphological phenotype of increased soma size mediated by the mTOR pathway. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | 4% |
Unknown | 24 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 9 | 36% |
Researcher | 8 | 32% |
Student > Doctoral Student | 2 | 8% |
Student > Postgraduate | 2 | 8% |
Professor | 1 | 4% |
Other | 2 | 8% |
Unknown | 1 | 4% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 9 | 36% |
Agricultural and Biological Sciences | 8 | 32% |
Medicine and Dentistry | 4 | 16% |
Immunology and Microbiology | 1 | 4% |
Materials Science | 1 | 4% |
Other | 0 | 0% |
Unknown | 2 | 8% |