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Planar Supported Membranes with Mobile SNARE Proteins and Quantitative Fluorescence Microscopy Assays to Study Synaptic Vesicle Fusion

Overview of attention for article published in Frontiers in Molecular Neuroscience, March 2017
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Title
Planar Supported Membranes with Mobile SNARE Proteins and Quantitative Fluorescence Microscopy Assays to Study Synaptic Vesicle Fusion
Published in
Frontiers in Molecular Neuroscience, March 2017
DOI 10.3389/fnmol.2017.00072
Pubmed ID
Authors

Volker Kiessling, Binyong Liang, Alex J. B. Kreutzberger, Lukas K. Tamm

Abstract

Synaptic vesicle membrane fusion, the process by which neurotransmitter gets released at the presynaptic membrane is mediated by a complex interplay between proteins and lipids. The realization that the lipid bilayer is not just a passive environment where other molecular players like SNARE proteins act, but is itself actively involved in the process, makes the development of biochemical and biophysical assays particularly challenging. We summarize in vitro assays that use planar supported membranes and fluorescence microscopy to address some of the open questions regarding the molecular mechanisms of SNARE-mediated membrane fusion. Most of the assays discussed in this mini-review were developed in our lab over the last 15 years. We emphasize the sample requirements that we found are important for the successful application of these methods.

X Demographics

X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 28%
Researcher 8 28%
Student > Master 3 10%
Student > Bachelor 2 7%
Student > Doctoral Student 2 7%
Other 2 7%
Unknown 4 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 13 45%
Agricultural and Biological Sciences 4 14%
Neuroscience 2 7%
Chemistry 2 7%
Physics and Astronomy 1 3%
Other 2 7%
Unknown 5 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 April 2017.
All research outputs
#14,055,371
of 22,961,203 outputs
Outputs from Frontiers in Molecular Neuroscience
#1,432
of 2,900 outputs
Outputs of similar age
#168,079
of 308,429 outputs
Outputs of similar age from Frontiers in Molecular Neuroscience
#55
of 105 outputs
Altmetric has tracked 22,961,203 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,900 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 47th percentile – i.e., 47% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 308,429 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 105 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.