Title |
Characterization of the Transcriptomes of Lgr5+ Hair Cell Progenitors and Lgr5- Supporting Cells in the Mouse Cochlea
|
---|---|
Published in |
Frontiers in Molecular Neuroscience, April 2017
|
DOI | 10.3389/fnmol.2017.00122 |
Pubmed ID | |
Authors |
Cheng Cheng, Luo Guo, Ling Lu, Xiaochen Xu, ShaSha Zhang, Junyan Gao, Muhammad Waqas, Chengwen Zhu, Yan Chen, Xiaoli Zhang, Chuanying Xuan, Xia Gao, Mingliang Tang, Fangyi Chen, Haibo Shi, Huawei Li, Renjie Chai |
Abstract |
Cochlear supporting cells (SCs) have been shown to be a promising resource for hair cell (HC) regeneration in the neonatal mouse cochlea. Previous studies have reported that Lgr5+ SCs can regenerate HCs both in vitro and in vivo and thus are considered to be inner ear progenitor cells. Lgr5+ progenitors are able to regenerate more HCs than Lgr5- SCs, and it is important to understand the mechanism behind the proliferation and HC regeneration of these progenitors. Here, we isolated Lgr5+ progenitors and Lgr5- SCs from Lgr5-EGFP-CreERT2/Sox2-CreERT2/Rosa26-tdTomato mice via flow cytometry. As expected, we found that Lgr5+ progenitors had significantly higher proliferation and HC regeneration ability than Lgr5- SCs. Next, we performed RNA-Seq to determine the gene expression profiles of Lgr5+ progenitors and Lgr5- SCs. We analyzed the genes that were enriched and differentially expressed in Lgr5+ progenitors and Lgr5- SCs, and we found 8 cell cycle genes, 9 transcription factors, and 24 cell signaling pathway genes that were uniquely expressed in one population but not the other. Last, we made a protein-protein interaction network to further analyze the role of these differentially expressed genes. In conclusion, we present a set of genes that might regulate the proliferation and HC regeneration ability of Lgr5+ progenitors, and these might serve as potential new therapeutic targets for HC regeneration. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Switzerland | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 38 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 18% |
Student > Ph. D. Student | 6 | 16% |
Student > Bachelor | 5 | 13% |
Student > Master | 5 | 13% |
Other | 2 | 5% |
Other | 3 | 8% |
Unknown | 10 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 11 | 29% |
Agricultural and Biological Sciences | 7 | 18% |
Neuroscience | 4 | 11% |
Medicine and Dentistry | 3 | 8% |
Physics and Astronomy | 1 | 3% |
Other | 2 | 5% |
Unknown | 10 | 26% |