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Mendeley readers
Attention Score in Context
| Title |
Regulation of Hippocampal 5-HT Release by P2X7 Receptors in Response to Optogenetic Stimulation of Median Raphe Terminals of Mice
|
|---|---|
| Published in |
Frontiers in Molecular Neuroscience, October 2017
|
| DOI | 10.3389/fnmol.2017.00325 |
| Pubmed ID | |
| Authors |
Flóra Gölöncsér, Mária Baranyi, Diána Balázsfi, Kornél Demeter, József Haller, Tamás F. F. Freund, Dóra Zelena, Beáta Sperlágh |
| Abstract |
Serotonergic and glutamatergic neurons of median raphe region (MRR) play a pivotal role in the modulation of affective and cognitive functions. These neurons synapse both onto themselves and remote cortical areas. P2X7 receptors (P2rx7) are ligand gated ion channels expressed by central presynaptic excitatory nerve terminals and involved in the regulation of neurotransmitter release. P2rx7s are implicated in various neuropsychiatric conditions such as schizophrenia and depression. Here we investigated whether 5-HT release released from the hippocampal terminals of MRR is subject to modulation by P2rx7s. To achieve this goal, an optogenetic approach was used to selectively activate subpopulation of serotonergic terminals derived from the MRR locally, and one of its target area, the hippocampus. Optogenetic activation of neurons in the MRR with 20 Hz was correlated with freezing and enhanced locomotor activity of freely moving mice and elevated extracellular levels of 5-HT, glutamate but not GABA in vivo. Similar optical stimulation (OS) significantly increased [(3)H]5-HT and [(3)H]glutamate release in acute MRR and hippocampal slices. We examined spatial and temporal patterns of [(3)H]5-HT release and the interaction between the serotonin and glutamate systems. Whilst [(3)H]5-HT release from MRR neurons was [Ca(2+)]o-dependent and sensitive to TTX, CNQX and DL-AP-5, release from hippocampal terminals was not affected by the latter drugs. Hippocampal [(3)H]5-HT released by electrical but not OS was subject to modulation by 5- HT1B/D receptors agonist sumatriptan (1 μM), whereas the selective 5-HT1A agonist buspirone (0.1 μM) was without effect. [(3)H]5-HT released by electrical and optical stimulation was decreased in mice genetically deficient in P2rx7s, and after perfusion with selective P2rx7 antagonists, JNJ-47965567 (0.1 μM), and AZ-10606120 (0.1 μM). Optical and electrical stimulation elevated the extracellular level of ATP. Our results demonstrate for the first time the modulation of 5-HT release from hippocampal MRR terminals by the endogenous activation of P2rx7s. P2rx7 mediated modulation of 5-HT release could contribute to various physiological and pathophysiological phenomena, related to hippocampal serotonergic transmission. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 20% |
| Mexico | 1 | 20% |
| Brazil | 1 | 20% |
| Switzerland | 1 | 20% |
| Unknown | 1 | 20% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 33 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 24% |
| Student > Bachelor | 6 | 18% |
| Researcher | 5 | 15% |
| Student > Master | 3 | 9% |
| Student > Doctoral Student | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 10 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 9 | 27% |
| Agricultural and Biological Sciences | 4 | 12% |
| Medicine and Dentistry | 3 | 9% |
| Biochemistry, Genetics and Molecular Biology | 2 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
| Other | 1 | 3% |
| Unknown | 12 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 October 2017.
All research outputs
#14,116,057
of 24,309,087 outputs
Outputs from Frontiers in Molecular Neuroscience
#1,335
of 3,171 outputs
Outputs of similar age
#161,538
of 328,696 outputs
Outputs of similar age from Frontiers in Molecular Neuroscience
#39
of 120 outputs
Altmetric has tracked 24,309,087 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,171 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one has gotten more attention than average, scoring higher than 57% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 328,696 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 120 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.