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Mendeley readers
Attention Score in Context
| Title |
Dynamic Arc SUMOylation and Selective Interaction with F-Actin-Binding Protein Drebrin A in LTP Consolidation In Vivo
|
|---|---|
| Published in |
Frontiers in Synaptic Neuroscience, May 2017
|
| DOI | 10.3389/fnsyn.2017.00008 |
| Pubmed ID | |
| Authors |
Rajeevkumar R. Nair, Sudarshan Patil, Adrian Tiron, Tambudzai Kanhema, Debabrata Panja, Lars Schiro, Kamil Parobczak, Grzegorz Wilczynski, Clive R. Bramham |
| Abstract |
Activity-regulatedcytoskeleton-associated protein (Arc) protein is implicated as a master regulator of long-term forms of synaptic plasticity and memory formation, but the mechanisms controlling Arc protein function are little known. Post-translation modification by small ubiquitin-like modifier (SUMO) proteins has emerged as a major mechanism for regulating protein-protein interactions and function. We first show in cell lines that ectopically expressed Arc undergoes mono-SUMOylation. The covalent addition of a single SUMO1 protein was confirmed by in vitro SUMOylation of immunoprecipitated Arc. To explore regulation of endogenous Arc during synaptic plasticity, we induced long-term potentiation (LTP) in the dentate gyrus of live anesthetized rats. Using coimmunoprecipitation of native proteins, we show that Arc synthesized during the maintenance phase of LTP undergoes dynamic mono-SUMO1-ylation. Levels of unmodified Arc increase in multiple subcellular fractions (cytosol, membrane, nuclear and cytoskeletal), whereas enhanced Arc SUMOylation was specific to the synaptoneurosomal and the cytoskeletal fractions. Dentate gyrus LTP consolidation requires a period of sustained Arc synthesis driven by brain-derived neurotrophic factor (BDNF) signaling. Local infusion of the BDNF scavenger, TrkB-Fc, during LTP maintenance resulted in rapid reversion of LTP, inhibition of Arc synthesis and loss of enhanced Arc SUMO1ylation. Furthermore, coimmunoprecipitation analysis showed that SUMO1-ylated Arc forms a complex with the F-actin-binding protein drebrin A, a major regulator of cytoskeletal dynamics in dendritic spines. Although Arc also interacted with dynamin 2, calcium/calmodulindependentprotein kinase II-beta (CaMKIIβ), and postsynaptic density protein-95 (PSD-95), these complexes lacked SUMOylated Arc. The results support a model in which newly synthesized Arc is SUMOylated and targeted for actin cytoskeletal regulation during in vivo LTP. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 85 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 1% |
| Unknown | 84 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 19 | 22% |
| Researcher | 14 | 16% |
| Student > Master | 12 | 14% |
| Student > Bachelor | 9 | 11% |
| Student > Doctoral Student | 4 | 5% |
| Other | 8 | 9% |
| Unknown | 19 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 22 | 26% |
| Biochemistry, Genetics and Molecular Biology | 20 | 24% |
| Agricultural and Biological Sciences | 14 | 16% |
| Environmental Science | 1 | 1% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 1% |
| Other | 7 | 8% |
| Unknown | 20 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 October 2017.
All research outputs
#13,548,595
of 22,965,074 outputs
Outputs from Frontiers in Synaptic Neuroscience
#210
of 415 outputs
Outputs of similar age
#158,901
of 310,763 outputs
Outputs of similar age from Frontiers in Synaptic Neuroscience
#5
of 5 outputs
Altmetric has tracked 22,965,074 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 415 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.0. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
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We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one.