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NanoLuc Luciferase – A Multifunctional Tool for High Throughput Antibody Screening

Overview of attention for article published in Frontiers in Pharmacology, February 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

Mentioned by

news
1 news outlet
twitter
7 X users
patent
3 patents

Readers on

mendeley
165 Mendeley
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Title
NanoLuc Luciferase – A Multifunctional Tool for High Throughput Antibody Screening
Published in
Frontiers in Pharmacology, February 2016
DOI 10.3389/fphar.2016.00027
Pubmed ID
Authors

Nicolas Boute, Peter Lowe, Sven Berger, Martine Malissard, Alain Robert, Michael Tesar

Abstract

Based on the recent development of NanoLuc luciferase (Nluc), a small (19 kDa), highly stable, ATP independent, bioluminescent protein, an extremely robust and ultra high sensitivity screening system has been developed whereby primary hits of therapeutic antibodies and antibody fragments could be characterized and quantified without purification. This system is very versatile allowing cellular and solid phase ELISA but also homogeneous BRET based screening assays, relative affinity determinations with competition ELISA and direct Western blotting. The new Nluc protein fusion represents a "swiss army knife solution" for today and future high throughput antibody drug screenings.

X Demographics

X Demographics

The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 165 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 <1%
Hong Kong 1 <1%
Czechia 1 <1%
Unknown 162 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 33 20%
Student > Ph. D. Student 27 16%
Student > Bachelor 27 16%
Student > Master 23 14%
Student > Postgraduate 6 4%
Other 18 11%
Unknown 31 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 49 30%
Agricultural and Biological Sciences 30 18%
Chemistry 11 7%
Pharmacology, Toxicology and Pharmaceutical Science 9 5%
Medicine and Dentistry 7 4%
Other 21 13%
Unknown 38 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 December 2023.
All research outputs
#1,845,822
of 24,978,429 outputs
Outputs from Frontiers in Pharmacology
#729
of 19,115 outputs
Outputs of similar age
#29,228
of 303,966 outputs
Outputs of similar age from Frontiers in Pharmacology
#9
of 111 outputs
Altmetric has tracked 24,978,429 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 19,115 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.2. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 303,966 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 111 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.