Title |
NanoLuc Luciferase – A Multifunctional Tool for High Throughput Antibody Screening
|
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Published in |
Frontiers in Pharmacology, February 2016
|
DOI | 10.3389/fphar.2016.00027 |
Pubmed ID | |
Authors |
Nicolas Boute, Peter Lowe, Sven Berger, Martine Malissard, Alain Robert, Michael Tesar |
Abstract |
Based on the recent development of NanoLuc luciferase (Nluc), a small (19 kDa), highly stable, ATP independent, bioluminescent protein, an extremely robust and ultra high sensitivity screening system has been developed whereby primary hits of therapeutic antibodies and antibody fragments could be characterized and quantified without purification. This system is very versatile allowing cellular and solid phase ELISA but also homogeneous BRET based screening assays, relative affinity determinations with competition ELISA and direct Western blotting. The new Nluc protein fusion represents a "swiss army knife solution" for today and future high throughput antibody drug screenings. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 29% |
France | 2 | 29% |
Switzerland | 1 | 14% |
Unknown | 2 | 29% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 6 | 86% |
Scientists | 1 | 14% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Japan | 1 | <1% |
Hong Kong | 1 | <1% |
Czechia | 1 | <1% |
Unknown | 162 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 33 | 20% |
Student > Ph. D. Student | 27 | 16% |
Student > Bachelor | 27 | 16% |
Student > Master | 23 | 14% |
Student > Postgraduate | 6 | 4% |
Other | 18 | 11% |
Unknown | 31 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 49 | 30% |
Agricultural and Biological Sciences | 30 | 18% |
Chemistry | 11 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 9 | 5% |
Medicine and Dentistry | 7 | 4% |
Other | 21 | 13% |
Unknown | 38 | 23% |