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Attention Score in Context
| Title |
Antiplatelet Agents Inhibit the Generation of Platelet-Derived Microparticles
|
|---|---|
| Published in |
Frontiers in Pharmacology, September 2016
|
| DOI | 10.3389/fphar.2016.00314 |
| Pubmed ID | |
| Authors |
Alice Giacomazzi, Maurizio Degan, Stefano Calabria, Alessandra Meneguzzi, Pietro Minuz |
| Abstract |
Platelet microparticles (PMPs) contribute to thrombogenesis but the effects of antiplatelet drugs on PMPs generation is undefined. The present study investigated the cellular events regulating PMPs shedding, testing in vitro platelet agonists and inhibitors. Platelet-rich plasma from healthy subjects was stimulated with arachidonic acid (AA), U46619, collagen type-I (10 and 1.5 μg/mL), epinephrine, ADP or TRAP-6 and pre-incubated with acetylsalicylic acid (ASA, 100 and 10 μmol/L), SQ-29,548, apyrase, PSB-0739, or eptifibatide. PMPs were detected by flow-cytometry using CD61 and annexin-V as fluorescent markers. Platelet agonists induced annexin V-positive PMPs shedding. The strongest response was to high concentration collagen. ADP-triggered PMPs shedding was dose-independent. ASA reduced PMPs induced by AA- (645, 347-2946 vs. 3061, 446-4901 PMPs/μL; median ad range, n = 9, P < 0.001), collagen 10 μg/mL (5317, 2027-15935 vs. 10252, 4187-46316 PMPs/μL; n = 13, P < 0.001), collagen 1.5 μg/mL (1078, 528-2820 vs. 1465, 582-5948 PMPs/μL; n = 21, P < 0.001) and TRAP-6 (2008, 1621-2495 vs. 2840, 2404-3031 PMPs/μL; n = 3, P < 0.01) but did not affect the response to epinephrine or ADP. The ADP scavenger apyrase reduced PMPs induced by U46619 (1256, 395-2908 vs. 3045, 1119-5494 PMPs/μL, n = 6, P < 0.05), collagen 1.5 μg/mL (1006, 780-1309 vs. 2422, 1839-3494 PMPs/μL, n = 3, P < 0.01) and TRAP-6 (904, 761-1224 vs. 2840, 2404-3031 PMPs/μL, n = 3, P < 0.01). The TP receptor antagonist SQ-29,548 and the P2Y12 receptor antagonist PSB-0739 markedly inhibited PMPs induced by low doses of collagen. Except for high-dose collagen, eptifibatide abolished agonist-induced PMPs release. Both TXA2 generation and ADP secretion are required as amplifiers of PMP shedding. The crucial role of the fibrinogen receptor and the collagen receptor in PMPs generation, independently of platelet aggregation, was identified. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 3% |
| Brazil | 1 | 3% |
| Unknown | 37 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 18% |
| Student > Bachelor | 6 | 15% |
| Researcher | 5 | 13% |
| Student > Ph. D. Student | 5 | 13% |
| Professor > Associate Professor | 3 | 8% |
| Other | 9 | 23% |
| Unknown | 4 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 11 | 28% |
| Biochemistry, Genetics and Molecular Biology | 6 | 15% |
| Agricultural and Biological Sciences | 5 | 13% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 8% |
| Immunology and Microbiology | 3 | 8% |
| Other | 5 | 13% |
| Unknown | 6 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 September 2016.
All research outputs
#20,341,859
of 22,888,307 outputs
Outputs from Frontiers in Pharmacology
#10,122
of 16,185 outputs
Outputs of similar age
#256,343
of 294,932 outputs
Outputs of similar age from Frontiers in Pharmacology
#94
of 167 outputs
Altmetric has tracked 22,888,307 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 16,185 research outputs from this source. They receive a mean Attention Score of 4.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 167 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.