Title |
Biodegradable Carriers for Delivery of VEGF Plasmid DNA for the Treatment of Critical Limb Ischemia
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Published in |
Frontiers in Pharmacology, August 2017
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DOI | 10.3389/fphar.2017.00528 |
Pubmed ID | |
Authors |
Guang Liu, Zhiwei Fang, Minglu Yuan, Weimin Li, Yunqi Yang, Mier Jiang, Yuanming Ouyang, Weien Yuan |
Abstract |
The safe and efficient delivery of therapeutic nucleic acid is a prerequisite for an effective DNA therapy. In this study, we condensed the low molecular weight polyethylenimine (PEI, 1.8k Da) with 2,6-pyridinedicarboxaldehyde (PDA), both of which are degradable in vivo, to synthesize a biodegradable polycationic material (PDAPEI) to deliver vascular endothelial growth factor (VEGF) plasmid DNA (pDNA). Particle size and zeta potential of this novel degradable PEI derivatives-pDNA nanoparticle were investigated and in vitro cytotoxicity was estimated on human umbilical vein endothelial cells (HUVECs). Using pDNA-encoding VEGF-A and green fluorescence protein (GFP), we also checked transfection efficiency of the vector (PDAPEI) and found its excellent performance at 40 w/w ratio. We successfully established peripheral ischemia animal model on C57/BL6J mice to evaluate the therapeutic effect of PDAPEI/pVEGF-A polyplex system on ischemic disease and a conclusion was made that PDAPEI is a promising gene vector in the treatment of peripheral ischemic artery disease (PAD). |
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Switzerland | 1 | 100% |
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Student > Bachelor | 4 | 24% |
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Professor > Associate Professor | 2 | 12% |
Student > Master | 1 | 6% |
Other | 0 | 0% |
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Other | 3 | 18% |
Unknown | 4 | 24% |