Title |
The possible impact of persistent virus infection on the function of the RNAi machinery in insects: a hypothesis
|
---|---|
Published in |
Frontiers in Physiology, January 2013
|
DOI | 10.3389/fphys.2013.00319 |
Pubmed ID | |
Authors |
Luc Swevers, Jozef Vanden Broeck, Guy Smagghe |
Abstract |
RNAi experiments in insects are characterized by great variability in efficiency; for instance beetles and locusts are very amenable to dsRNA-mediated gene silencing, while other insect groups, most notably lepidopterans, are more refractory to RNAi. Several factors can be forwarded that could affect the efficiency of RNAi, such as the composition and function of the intracellular RNAi machinery, the mechanism of dsRNA uptake, the presence of dsRNA- and siRNA-degrading enzymes and non-specific activation of the innate immune response. In this essay, we investigate the evidence whether persistent infection with RNA viruses could be a major factor that affects the response to exogenous dsRNA in insects. The occurrence of RNA viruses in different insect groups will be discussed, as well as several mechanisms by which viruses could interfere with the process of RNAi. Finally, the impact of RNA virus infection on the design of dsRNA-based insect control strategies will be considered. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Switzerland | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Slovenia | 1 | 1% |
Unknown | 99 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 23 | 23% |
Researcher | 21 | 21% |
Student > Master | 17 | 17% |
Student > Bachelor | 13 | 13% |
Professor | 7 | 7% |
Other | 8 | 8% |
Unknown | 11 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 56 | 56% |
Biochemistry, Genetics and Molecular Biology | 19 | 19% |
Engineering | 3 | 3% |
Nursing and Health Professions | 1 | 1% |
Veterinary Science and Veterinary Medicine | 1 | 1% |
Other | 8 | 8% |
Unknown | 12 | 12% |