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Detecting cardiac contractile activity in the early mouse embryo using multiple modalities

Overview of attention for article published in Frontiers in Physiology, January 2015
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Title
Detecting cardiac contractile activity in the early mouse embryo using multiple modalities
Published in
Frontiers in Physiology, January 2015
DOI 10.3389/fphys.2014.00508
Pubmed ID
Authors

Chiann-Mun Chen, António M. A. Miranda, Gil Bub, Shankar Srinivas

Abstract

The heart is one of the first organs to develop during mammalian embryogenesis. In the mouse, it starts to form shortly after gastrulation, and is derived primarily from embryonic mesoderm. The embryonic heart is unique in having to perform a mechanical contractile function while undergoing complex morphogenetic remodeling. Approaches to imaging the morphogenesis and contractile activity of the developing heart are important in understanding not only how this remodeling is controlled but also the origin of congenital heart defects (CHDs). Here, we describe approaches for visualizing contractile activity in the developing mouse embryo, using brightfield time lapse microscopy and confocal microscopy of calcium transients. We describe an algorithm for enhancing this image data and quantifying contractile activity from it. Finally we describe how atomic force microscopy can be used to record contractile activity prior to it being microscopically visible.

X Demographics

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 3 14%
Student > Master 3 14%
Student > Ph. D. Student 3 14%
Professor 2 10%
Researcher 2 10%
Other 1 5%
Unknown 7 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 29%
Biochemistry, Genetics and Molecular Biology 2 10%
Engineering 2 10%
Medicine and Dentistry 2 10%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Other 0 0%
Unknown 8 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 January 2015.
All research outputs
#17,736,409
of 22,776,824 outputs
Outputs from Frontiers in Physiology
#7,117
of 13,560 outputs
Outputs of similar age
#241,465
of 352,357 outputs
Outputs of similar age from Frontiers in Physiology
#57
of 115 outputs
Altmetric has tracked 22,776,824 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,560 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.5. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,357 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 115 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.