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Flow cytometry and K-mer analysis estimates of the genome sizes of Bemisia tabaci B and Q (Hemiptera: Aleyrodidae)

Overview of attention for article published in Frontiers in Physiology, May 2015
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Title
Flow cytometry and K-mer analysis estimates of the genome sizes of Bemisia tabaci B and Q (Hemiptera: Aleyrodidae)
Published in
Frontiers in Physiology, May 2015
DOI 10.3389/fphys.2015.00144
Pubmed ID
Authors

Li T. Guo, Shao L. Wang, Qing J. Wu, Xu G. Zhou, Wen Xie, You J. Zhang

Abstract

The genome sizes of the B- and Q-types of the whitefly Bemisia tabaci (Gennnadius) were estimated using flow cytometry (Drosophila melanogaster as the DNA reference standard and propidium iodide (PI) as the fluorochrome) and k-mer analysis. For flow cytometry, the mean nuclear DNA content was 0.686 pg for B-type males, 1.392 pg for B-type females, 0.680 pg for Q-type males, and 1.306 pg for Q-type females. Based on the relationship between DNA content and genome size (1 pg DNA = 980 Mbp), the haploid genome size of B. tabaci ranged from 640 to 682 Mbp. For k-mer analysis, genome size of B-type by two methods were consistent highly, but the k-mer depth distribution graph of Q-type was not enough perfect and the genome size was estimated about 60 M larger than its flow cytometry result. These results corroborate previous reports of genome size based on karyotype analysis and chromosome counting. However, these estimates differ from previous flow cytometry estimates, probably because of differences in the DNA reference standard and dyeing time, which were superior in the current study. For Q-type genome size difference by two method, some discussion were also stated, and all these results represent a useful foundation for B. tabaci genomics research.

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Geographical breakdown

Country Count As %
Colombia 1 2%
Portugal 1 2%
France 1 2%
Germany 1 2%
Unknown 48 92%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 23%
Researcher 10 19%
Student > Ph. D. Student 10 19%
Student > Doctoral Student 6 12%
Student > Bachelor 2 4%
Other 4 8%
Unknown 8 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 19 37%
Biochemistry, Genetics and Molecular Biology 19 37%
Neuroscience 2 4%
Computer Science 2 4%
Environmental Science 1 2%
Other 0 0%
Unknown 9 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 May 2015.
All research outputs
#19,015,492
of 23,577,654 outputs
Outputs from Frontiers in Physiology
#8,538
of 14,284 outputs
Outputs of similar age
#194,480
of 267,561 outputs
Outputs of similar age from Frontiers in Physiology
#54
of 86 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 14,284 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.7. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
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We're also able to compare this research output to 86 others from the same source and published within six weeks on either side of this one. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.