Title |
RNAi Efficiency, Systemic Properties, and Novel Delivery Methods for Pest Insect Control: What We Know So Far
|
---|---|
Published in |
Frontiers in Physiology, November 2016
|
DOI | 10.3389/fphys.2016.00553 |
Pubmed ID | |
Authors |
Mallikarjuna R. Joga, Moises J. Zotti, Guy Smagghe, Olivier Christiaens |
Abstract |
In recent years, the research on the potential of using RNA interference (RNAi) to suppress crop pests has made an outstanding growth. However, given the variability of RNAi efficiency that is observed in many insects, the development of novel approaches toward insect pest management using RNAi requires first to unravel factors behind the efficiency of dsRNA-mediated gene silencing. In this review, we explore essential implications and possibilities to increase RNAi efficiency by delivery of dsRNA through non-transformative methods. We discuss factors influencing the RNAi mechanism in insects and systemic properties of dsRNA. Finally, novel strategies to deliver dsRNA are discussed, including delivery by symbionts, plant viruses, trunk injections, root soaking, and transplastomic plants. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 25% |
Belgium | 1 | 25% |
Switzerland | 1 | 25% |
Unknown | 1 | 25% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 75% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | <1% |
Germany | 1 | <1% |
Unknown | 477 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 88 | 18% |
Student > Bachelor | 67 | 14% |
Researcher | 62 | 13% |
Student > Master | 60 | 13% |
Student > Doctoral Student | 22 | 5% |
Other | 67 | 14% |
Unknown | 113 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 177 | 37% |
Biochemistry, Genetics and Molecular Biology | 96 | 20% |
Unspecified | 19 | 4% |
Environmental Science | 10 | 2% |
Chemistry | 10 | 2% |
Other | 33 | 7% |
Unknown | 134 | 28% |