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Effects of Dietary Phosphorus Level on the Expression of Calcium and Phosphorus Transporters in Laying Hens

Overview of attention for article published in Frontiers in Physiology, May 2018
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Title
Effects of Dietary Phosphorus Level on the Expression of Calcium and Phosphorus Transporters in Laying Hens
Published in
Frontiers in Physiology, May 2018
DOI 10.3389/fphys.2018.00627
Pubmed ID
Authors

Peng Li, Rongmei Wang, Hongchao Jiao, Xiaojuan Wang, Jingpeng Zhao, Hai Lin

Abstract

The transport of calcium and phosphorus is mainly relied on their corresponding transporters. The aim of this study was to determine the effect of dietary phosphorus level on the expression of the relevant calcium and phosphorus transporters in laying hens, which has a large amount of calcium and phosphorus input from intestine and output from kidney and eggshell gland. Thirty-six 25-week-old Hy-line Brown hens were fed diets with different available phosphorus level (AP, 0.15, 0.41, and 0.82%), respectively. The expression of phosphorus transporters type IIa and type IIb Na/Pi co-transporter (NPt2a, NPt2b), calcium transporter calbindin-D28k (CaBP-D28k), and plasma membrane Ca ATPase 1b (PMCA1b) were measured in small intestine, kidney, and eggshell gland by RT-PCR and western blot. The results showed that serum calcitriol and PTH concentrations were not affected (P > 0.05) by dietary AP levels. Duodenum had the highest mRNA and protein expression level of NPt2b than jejunum and ileum (P < 0.05). The protein expression abundance of CaBP-D28k and PMCA1b were higher in duodenum than that in jejunum and ileum (P < 0.05). 0.15%-AP diet upregulated the ileal mRNA expression level of NPt2b and renal mRNA expression level of NPt2a (P < 0.05), while downregulated the protein abundance of NPt2b and CaBP-D28k mRNA expression in shell gland (P < 0.05). In conclusion, both the Ca and P transporters were highly expressed in duodenum. Low AP diet decreased protein expression abundance of NPt2b in duodenum while upregulated the mRNA expression level of NPt2a in kidney. The result suggests that both the phosphorus absorption in proximal intestine and its reabsorption in kidney are involved in the adaption to low AP diet.

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The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 23%
Researcher 3 10%
Student > Master 3 10%
Student > Bachelor 2 7%
Other 1 3%
Other 0 0%
Unknown 14 47%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 37%
Veterinary Science and Veterinary Medicine 1 3%
Nursing and Health Professions 1 3%
Biochemistry, Genetics and Molecular Biology 1 3%
Immunology and Microbiology 1 3%
Other 1 3%
Unknown 14 47%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 June 2018.
All research outputs
#18,135,049
of 23,298,349 outputs
Outputs from Frontiers in Physiology
#7,364
of 14,025 outputs
Outputs of similar age
#240,045
of 331,376 outputs
Outputs of similar age from Frontiers in Physiology
#277
of 482 outputs
Altmetric has tracked 23,298,349 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 14,025 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.6. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 331,376 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 482 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.