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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Modification of Seed Oil Composition in Arabidopsis by Artificial microRNA-Mediated Gene Silencing
|
|---|---|
| Published in |
Frontiers in Plant Science, January 2012
|
| DOI | 10.3389/fpls.2012.00168 |
| Pubmed ID | |
| Authors |
Srinivas Belide, James Robertson Petrie, Pushkar Shrestha, Surinder Pal Singh |
| Abstract |
Various post transcriptional gene silencing strategies have been developed and exploited to study gene function or engineer disease resistance. The recently developed artificial microRNA strategy is an alternative method of effectively silencing target genes. The Δ12-desaturase (FAD2), Fatty acid elongase (FAE1), and Fatty acyl-ACP thioesterase B (FATB) were targeted with amiR159b-based constructs in Arabidopsisthaliana to evaluate changes in oil composition when expressed with the seed-specific Brassica napus truncated napin (FP1) promoter. Fatty acid profiles from transgenic homozygous seeds reveal that the targeted genes were silenced. The down-regulation of the AtFAD-2 gene substantially increased oleic acid from the normal levels of ∼15% to as high as 63.3 and reduced total PUFA content (18:2(Δ9,12) + 18:3(Δ9,12,15) + 20:2(Δ11,14) + 20:3(Δ11,14,17)) from 46.8 to 4.8%. Δ12-desaturase activity was reduced to levels as low as those in the null fad-2-1 and fad-2-2 mutants. Silencing of the FAE1 gene resulted in the reduction of eicosenoic acid (20:1(Δ11)) to 1.9 from 15.4% and silencing of FATB resulted in the reduction of palmitic acid (16:0) to 4.4% from 8.0%. Reduction in FATB activity is comparable with a FATB knock-out mutant. These results demonstrate for the first time amiR159b constructs targeted against three endogenous seed-expressed genes are clearly able to down-regulate and generate genotypic changes that are inherited stably over three generations. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 65 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 17% |
| Researcher | 11 | 17% |
| Student > Master | 10 | 15% |
| Student > Bachelor | 4 | 6% |
| Professor > Associate Professor | 4 | 6% |
| Other | 13 | 20% |
| Unknown | 12 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 36 | 55% |
| Biochemistry, Genetics and Molecular Biology | 13 | 20% |
| Unspecified | 1 | 2% |
| Environmental Science | 1 | 2% |
| Immunology and Microbiology | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 12 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2018.
All research outputs
#7,173,115
of 22,675,759 outputs
Outputs from Frontiers in Plant Science
#4,407
of 19,843 outputs
Outputs of similar age
#67,823
of 244,088 outputs
Outputs of similar age from Frontiers in Plant Science
#36
of 195 outputs
Altmetric has tracked 22,675,759 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 19,843 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 244,088 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 195 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.