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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Sequencing of Chloroplast Genome Using Whole Cellular DNA and Solexa Sequencing Technology
|
|---|---|
| Published in |
Frontiers in Plant Science, January 2012
|
| DOI | 10.3389/fpls.2012.00243 |
| Pubmed ID | |
| Authors |
Jian Wu, Bo Liu, Feng Cheng, Nirala Ramchiary, Su Ryun Choi, Yong Pyo Lim, Xiao-Wu Wang |
| Abstract |
Sequencing of the chloroplast (cp) genome using traditional sequencing methods has been difficult because of its size (>120 kb) and the complicated procedures required to prepare templates. To explore the feasibility of sequencing the cp genome using DNA extracted from whole cells and Solexa sequencing technology, we sequenced whole cellular DNA isolated from leaves of three Brassicarapa accessions with one lane per accession. In total, 246, 362, and 361 Mb sequence data were generated for the three accessions Chiifu-401-42, Z16, and FT, respectively. Micro-reads were assembled by reference-guided assembly using the cpDNA sequences of B. rapa, Arabidopsis thaliana, and Nicotiana tabacum. We achieved coverage of more than 99.96% of the cp genome in the three tested accessions using the B. rapa sequence as the reference. When A. thaliana or N. tabacum sequences were used as references, 99.7-99.8 or 95.5-99.7% of the B. rapa cp genome was covered, respectively. These results demonstrated that sequencing of whole cellular DNA isolated from young leaves using the Illumina Genome Analyzer is an efficient method for high-throughput sequencing of cp genome. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 83 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 1% |
| Netherlands | 1 | 1% |
| Sweden | 1 | 1% |
| Brazil | 1 | 1% |
| Unknown | 79 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 19 | 23% |
| Student > Ph. D. Student | 17 | 20% |
| Student > Master | 8 | 10% |
| Student > Bachelor | 6 | 7% |
| Professor > Associate Professor | 4 | 5% |
| Other | 14 | 17% |
| Unknown | 15 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 38 | 46% |
| Biochemistry, Genetics and Molecular Biology | 17 | 20% |
| Environmental Science | 2 | 2% |
| Chemistry | 2 | 2% |
| Social Sciences | 2 | 2% |
| Other | 5 | 6% |
| Unknown | 17 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 November 2012.
All research outputs
#17,670,751
of 22,685,926 outputs
Outputs from Frontiers in Plant Science
#11,819
of 19,871 outputs
Outputs of similar age
#191,346
of 244,123 outputs
Outputs of similar age from Frontiers in Plant Science
#84
of 195 outputs
Altmetric has tracked 22,685,926 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 19,871 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 244,123 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 195 others from the same source and published within six weeks on either side of this one. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.