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Comparative sequence analysis of nitrogen fixation-related genes in six legumes

Overview of attention for article published in Frontiers in Plant Science, January 2013
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Title
Comparative sequence analysis of nitrogen fixation-related genes in six legumes
Published in
Frontiers in Plant Science, January 2013
DOI 10.3389/fpls.2013.00300
Pubmed ID
Authors

Dong Hyun Kim, Swathi Parupalli, Sarwar Azam, Suk-Ha Lee, Rajeev K. Varshney

Abstract

Legumes play an important role as food and forage crops in international agriculture especially in developing countries. Legumes have a unique biological process called nitrogen fixation (NF) by which they convert atmospheric nitrogen to ammonia. Although legume genomes have undergone polyploidization, duplication and divergence, NF-related genes, because of their essential functional role for legumes, might have remained conserved. To understand the relationship of divergence and evolutionary processes in legumes, this study analyzes orthologs and paralogs for selected 20 NF-related genes by using comparative genomic approaches in six legumes i.e., Medicago truncatula (Mt), Cicer arietinum, Lotus japonicus, Cajanus cajan (Cc), Phaseolus vulgaris (Pv), and Glycine max (Gm). Subsequently, sequence distances, numbers of synonymous substitutions per synonymous site (Ks) and non-synonymous substitutions per non-synonymous site (Ka) between orthologs and paralogs were calculated and compared across legumes. These analyses suggest the closest relationship between Gm and Cc and the highest distance between Mt and Pv in six legumes. Ks proportional plots clearly showed ancient genome duplication in all legumes, whole genome duplication event in Gm and also speciation pattern in different legumes. This study also reports some interesting observations e.g., no peak at Ks 0.4 in Gm-Gm, location of two independent genes next to each other in Mt and low Ks values for outparalogs for three genes as compared to other 12 genes. In summary, this study underlines the importance of NF-related genes and provides important insights in genome organization and evolutionary aspects of six legume species analyzed.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 6%
Netherlands 1 1%
France 1 1%
Austria 1 1%
Italy 1 1%
United Kingdom 1 1%
Brazil 1 1%
Unknown 62 86%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 24 33%
Researcher 19 26%
Student > Master 7 10%
Professor > Associate Professor 3 4%
Student > Bachelor 2 3%
Other 7 10%
Unknown 10 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 46 64%
Biochemistry, Genetics and Molecular Biology 5 7%
Environmental Science 3 4%
Computer Science 3 4%
Engineering 2 3%
Other 1 1%
Unknown 12 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 August 2013.
All research outputs
#13,388,742
of 22,716,996 outputs
Outputs from Frontiers in Plant Science
#6,590
of 19,961 outputs
Outputs of similar age
#158,277
of 280,757 outputs
Outputs of similar age from Frontiers in Plant Science
#111
of 517 outputs
Altmetric has tracked 22,716,996 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 19,961 research outputs from this source. They receive a mean Attention Score of 4.0. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,757 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 517 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.