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Optimization and effects of different culture conditions on growth of Halomicronema hongdechloris – a filamentous cyanobacterium containing chlorophyll f

Overview of attention for article published in Frontiers in Plant Science, January 2014
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Title
Optimization and effects of different culture conditions on growth of Halomicronema hongdechloris – a filamentous cyanobacterium containing chlorophyll f
Published in
Frontiers in Plant Science, January 2014
DOI 10.3389/fpls.2014.00067
Pubmed ID
Authors

Yaqiong Li, Yuankui Lin, Patrick C. Loughlin, Min Chen

Abstract

A chlorophyll f containing cyanobacterium, Halomicronema hongdechloris (H. hongdechloris) was isolated from a stromatolite cyanobacterial community. The extremely slow growth rate of H. hongdechloris has hindered research on this newly isolated cyanobacterium and the investigation of chlorophyll f-photosynthesis. Therefore, optimizing H. hongdechloris culture conditions has become an essential requirement for future research. This work investigated the effects of various culture conditions, essential nutrients and light environments to determine the optimal growth conditions for H. hongdechloris and the biosynthetic rate of chlorophyll f. Based on the total chlorophyll concentration, an optimal growth rate of 0.22 ± 0.02 day(-1)(doubling time: 3.1 ± 0.3 days) was observed when cells were grown under continuous illumination with far-red light with an intensity of 20 μE at 32°C in modified K + ES seawater (pH 8.0) with additional nitrogen and phosphor supplements. High performance liquid chromatography on H. hongdechloris pigments confirmed that chlorophyll a is the major chlorophyll and chlorophyll f constitutes ~10% of the total chlorophyll from cells grown under far-red light. Fluorescence confocal image analysis demonstrated changes of photosynthetic membranes and the distribution of photopigments in response to different light conditions. The total photosynthetic oxygen evolution yield per cell showed no changes under different light conditions, which confirms the involvement of chlorophyll f in oxygenic photosynthesis. The implications of the presence of chlorophyll f in H. hongdechloris and its relationship with the ambient light environment are discussed.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 102 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 102 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 18 18%
Student > Ph. D. Student 16 16%
Student > Bachelor 12 12%
Researcher 9 9%
Other 8 8%
Other 18 18%
Unknown 21 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 30 29%
Biochemistry, Genetics and Molecular Biology 26 25%
Environmental Science 6 6%
Immunology and Microbiology 5 5%
Unspecified 4 4%
Other 8 8%
Unknown 23 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 February 2014.
All research outputs
#22,758,309
of 25,373,627 outputs
Outputs from Frontiers in Plant Science
#19,712
of 24,597 outputs
Outputs of similar age
#280,467
of 319,271 outputs
Outputs of similar age from Frontiers in Plant Science
#44
of 85 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,597 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 319,271 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 85 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.