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The non-homologous end-joining pathway is involved in stable transformation in rice

Overview of attention for article published in Frontiers in Plant Science, October 2014
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1 Facebook page

Citations

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47 Mendeley
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Title
The non-homologous end-joining pathway is involved in stable transformation in rice
Published in
Frontiers in Plant Science, October 2014
DOI 10.3389/fpls.2014.00560
Pubmed ID
Authors

Hiroaki Saika, Ayako Nishizawa-Yokoi, Seiichi Toki

Abstract

Stable transformation with T-DNA needs the coordinated activities of many proteins derived from both host plant cells and Agrobacterium. In dicot plants, including Arabidopsis, it has been suggested that non-homologous end-joining (NHEJ)-one of the main DNA double-strand break repair pathways-is involved in the T-DNA integration step that is crucial to stable transformation. However, how this pathway is involved remains unclear as results with NHEJ mutants in Arabidopsis have given inconsistent results. Recently, a system for visualization of stable expression of genes located on T-DNA has been established in rice callus. Stable expression was shown to be reduced significantly in NHEJ knock-down rice calli, suggesting strongly that NHEJ is involved in Agrobacterium-mediated stable transformation in rice. Since rice transformation is now efficient and reproducible, rice is a good model plant in which to elucidate the molecular mechanisms of T-DNA integration.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Belgium 1 2%
Unknown 46 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 21%
Researcher 9 19%
Student > Bachelor 7 15%
Student > Postgraduate 4 9%
Student > Master 4 9%
Other 5 11%
Unknown 8 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 24 51%
Biochemistry, Genetics and Molecular Biology 12 26%
Arts and Humanities 1 2%
Unspecified 1 2%
Business, Management and Accounting 1 2%
Other 1 2%
Unknown 7 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 October 2014.
All research outputs
#15,307,723
of 22,766,595 outputs
Outputs from Frontiers in Plant Science
#10,780
of 20,063 outputs
Outputs of similar age
#150,420
of 258,403 outputs
Outputs of similar age from Frontiers in Plant Science
#103
of 197 outputs
Altmetric has tracked 22,766,595 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,063 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 258,403 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 197 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.