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Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by…

Overview of attention for article published in Frontiers in Plant Science, September 2015
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Title
Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens
Published in
Frontiers in Plant Science, September 2015
DOI 10.3389/fpls.2015.00727
Pubmed ID
Authors

Abang Masli Dayang Izawati, Mat Yunus Abdul Masani, Ismail Ismanizan, Ghulam Kadir Ahmad Parveez

Abstract

DOG(R)1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOG(R)1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l(-1) 2-deoxyglucose (2-DOG) as the selection agent. 2-DOG resistant tissues were regenerated into whole plantlets on various regeneration media containing the same concentration of 2-DOG. The plantlets were later transferred into soil and grown in a biosafety screenhouse. PCR and subsequently Southern blot analyses were carried out to confirm the integration of the transgene in the plantlets. A transformation efficiency of about 1.0% was obtained using DOG(R)1 gene into the genome of oil palm. This result demonstrates the potential of using combination of DOG(R)1 gene and 2-DOG for regenerating transgenic oil palm.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Indonesia 1 3%
United Kingdom 1 3%
Unknown 31 94%

Demographic breakdown

Readers by professional status Count As %
Student > Master 8 24%
Student > Bachelor 7 21%
Researcher 7 21%
Student > Ph. D. Student 5 15%
Professor 1 3%
Other 2 6%
Unknown 3 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 55%
Biochemistry, Genetics and Molecular Biology 10 30%
Business, Management and Accounting 1 3%
Unknown 4 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 September 2015.
All research outputs
#20,292,660
of 22,829,083 outputs
Outputs from Frontiers in Plant Science
#16,033
of 20,139 outputs
Outputs of similar age
#230,653
of 274,809 outputs
Outputs of similar age from Frontiers in Plant Science
#258
of 354 outputs
Altmetric has tracked 22,829,083 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,139 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 354 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.