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Internal transcribed spacer 2 barcode: a good tool for identifying Acanthopanacis cortex

Overview of attention for article published in Frontiers in Plant Science, October 2015
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Title
Internal transcribed spacer 2 barcode: a good tool for identifying Acanthopanacis cortex
Published in
Frontiers in Plant Science, October 2015
DOI 10.3389/fpls.2015.00840
Pubmed ID
Authors

Sha Zhao, Xiaochen Chen, Jingyuan Song, Xiaohui Pang, Shilin Chen

Abstract

Acanthopanacis cortex has been used in clinical applications for a long time. Considering some historical and geographical factors, Acanthopanacis cortex is easily confused with other herbs in medicine markets, thereby causing potential safety issues. In this study, we used the internal transcribed spacer 2 (ITS2) barcode to identify 69 samples belonging to six species, including Acanthopanacis cortex and its adulterants. The nearest distance, single-nucleotide polymorphisms (SNPs), and neighbor-joining (NJ) tree methods were used to evaluate the identification ability of the ITS2 barcode. According to the kimura-2-parameter model, the intraspecific distance of Eleutherococcus nodiflorus ITS2 sequences ranged from 0 to 0.0132. The minimum interspecific distance between E. nodiflorus and E. giraldii was 0.0221, which was larger than the maximum intraspecific distance of E. nodiflorus. Three stable SNPs in ITS2 can be used to distinguish Acanthopanacis cortex and its closely related species. The NJ tree indicated that the Acanthopanacis cortex samples clustered into one clade, which can be distinguished clearly from the adulterants of this herb. A secondary structure of ITS2 provided another dimensionality to identify species. In conclusion, the ITS2 barcode effectively identifies Acanthopanacis cortex, and DNA barcoding is a convenient tool for medicine market supervision.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 27%
Student > Master 6 27%
Student > Bachelor 1 5%
Lecturer 1 5%
Student > Ph. D. Student 1 5%
Other 0 0%
Unknown 7 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 50%
Agricultural and Biological Sciences 2 9%
Veterinary Science and Veterinary Medicine 1 5%
Medicine and Dentistry 1 5%
Unknown 7 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2015.
All research outputs
#20,293,238
of 22,829,683 outputs
Outputs from Frontiers in Plant Science
#16,039
of 20,146 outputs
Outputs of similar age
#233,369
of 278,190 outputs
Outputs of similar age from Frontiers in Plant Science
#267
of 363 outputs
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