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Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress

Overview of attention for article published in Frontiers in Plant Science, November 2015
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Title
Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress
Published in
Frontiers in Plant Science, November 2015
DOI 10.3389/fpls.2015.00928
Pubmed ID
Authors

Zuzana Krčková, Jitka Brouzdová, Michal Daněk, Daniela Kocourková, Dominique Rainteau, Eric Ruelland, Olga Valentová, Přemysl Pejchar, Jan Martinec

Abstract

The Arabidopsis non-specific phospholipase C (NPC) protein family is encoded by the genes NPC1 - NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Czechia 1 3%
Unknown 34 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 34%
Student > Bachelor 5 14%
Researcher 4 11%
Student > Doctoral Student 3 9%
Student > Master 3 9%
Other 4 11%
Unknown 4 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 17 49%
Biochemistry, Genetics and Molecular Biology 13 37%
Philosophy 1 3%
Chemistry 1 3%
Unknown 3 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 November 2015.
All research outputs
#18,430,119
of 22,832,057 outputs
Outputs from Frontiers in Plant Science
#13,748
of 20,146 outputs
Outputs of similar age
#205,240
of 285,322 outputs
Outputs of similar age from Frontiers in Plant Science
#225
of 358 outputs
Altmetric has tracked 22,832,057 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,146 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 285,322 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 358 others from the same source and published within six weeks on either side of this one. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.