Title |
Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress
|
---|---|
Published in |
Frontiers in Plant Science, November 2015
|
DOI | 10.3389/fpls.2015.00928 |
Pubmed ID | |
Authors |
Zuzana Krčková, Jitka Brouzdová, Michal Daněk, Daniela Kocourková, Dominique Rainteau, Eric Ruelland, Olga Valentová, Přemysl Pejchar, Jan Martinec |
Abstract |
The Arabidopsis non-specific phospholipase C (NPC) protein family is encoded by the genes NPC1 - NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Switzerland | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Czechia | 1 | 3% |
Unknown | 34 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 12 | 34% |
Student > Bachelor | 5 | 14% |
Researcher | 4 | 11% |
Student > Doctoral Student | 3 | 9% |
Student > Master | 3 | 9% |
Other | 4 | 11% |
Unknown | 4 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 17 | 49% |
Biochemistry, Genetics and Molecular Biology | 13 | 37% |
Philosophy | 1 | 3% |
Chemistry | 1 | 3% |
Unknown | 3 | 9% |