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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Isolation of Plant Photosystem II Complexes by Fractional Solubilization
|
|---|---|
| Published in |
Frontiers in Plant Science, December 2015
|
| DOI | 10.3389/fpls.2015.01100 |
| Pubmed ID | |
| Authors |
Patrycja Haniewicz, Davide Floris, Domenica Farci, Joanna Kirkpatrick, Maria C. Loi, Claudia Büchel, Matthias Bochtler, Dario Piano |
| Abstract |
Photosystem II (PSII) occurs in different forms and supercomplexes in thylakoid membranes. Using a transplastomic strain of Nicotiana tabacum histidine tagged on the subunit PsbE, we have previously shown that a mild extraction protocol with β-dodecylmaltoside enriches PSII characteristic of lamellae and grana margins. Here, we characterize residual granal PSII that is not extracted by this first solubilization step. Using affinity purification, we demonstrate that this PSII fraction consists of PSII-LHCII mega- and supercomplexes, PSII dimers, and PSII monomers, which were separated by gel filtration and functionally characterized. Our findings represent an alternative demonstration of different PSII populations in thylakoid membranes, and they make it possible to prepare PSII-LHCII supercomplexes in high yield. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 4% |
| Unknown | 26 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 22% |
| Student > Ph. D. Student | 6 | 22% |
| Student > Master | 3 | 11% |
| Student > Doctoral Student | 1 | 4% |
| Other | 1 | 4% |
| Other | 2 | 7% |
| Unknown | 8 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 33% |
| Biochemistry, Genetics and Molecular Biology | 7 | 26% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Environmental Science | 1 | 4% |
| Chemistry | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 8 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 December 2015.
All research outputs
#20,298,249
of 22,835,198 outputs
Outputs from Frontiers in Plant Science
#16,044
of 20,148 outputs
Outputs of similar age
#326,305
of 388,835 outputs
Outputs of similar age from Frontiers in Plant Science
#322
of 418 outputs
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So far Altmetric has tracked 20,148 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 418 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.