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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Seed-Specific Expression of Spider Silk Protein Multimers Causes Long-Term Stability
|
|---|---|
| Published in |
Frontiers in Plant Science, January 2016
|
| DOI | 10.3389/fpls.2016.00006 |
| Pubmed ID | |
| Authors |
Nicola Weichert, Valeska Hauptmann, Christine Helmold, Udo Conrad |
| Abstract |
Seeds enable plants to germinate and to grow in situations of limited availability of nutrients. The stable storage of different seed proteins is a remarkable presumption for successful germination and growth. These strategies have been adapted and used in several molecular farming projects. In this study, we explore the benefits of seed-based expression to produce the high molecular weight spider silk protein FLAG using intein-based trans-splicing. Multimers larger than 460 kDa in size are routinely produced, which is above the native size of the FLAG protein. The storage of seeds for 8 weeks and 1 year at an ambient temperature of 15°C does not influence the accumulation level. Even the extended storage time does not influence the typical pattern of multimerized bands. These results show that seeds are the method of choice for stable accumulation of products of complex transgenes and have the capability for long-term storage at moderate conditions, an important feature for the development of suitable downstream processes. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 1 | 25% |
| United States | 1 | 25% |
| Germany | 1 | 25% |
| Switzerland | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 2% |
| Unknown | 46 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 21% |
| Researcher | 9 | 19% |
| Student > Bachelor | 6 | 13% |
| Student > Master | 3 | 6% |
| Other | 2 | 4% |
| Other | 6 | 13% |
| Unknown | 11 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 16 | 34% |
| Agricultural and Biological Sciences | 11 | 23% |
| Materials Science | 4 | 9% |
| Computer Science | 1 | 2% |
| Environmental Science | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 12 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 October 2016.
All research outputs
#13,964,379
of 22,842,950 outputs
Outputs from Frontiers in Plant Science
#7,287
of 20,166 outputs
Outputs of similar age
#201,313
of 396,721 outputs
Outputs of similar age from Frontiers in Plant Science
#136
of 496 outputs
Altmetric has tracked 22,842,950 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,166 research outputs from this source. They receive a mean Attention Score of 4.0. This one has gotten more attention than average, scoring higher than 60% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 396,721 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 496 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.