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Development and Identification of SSR Markers Associated with Starch Properties and β-Carotene Content in the Storage Root of Sweet Potato (Ipomoea batatas L.)

Overview of attention for article published in Frontiers in Plant Science, March 2016
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

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Title
Development and Identification of SSR Markers Associated with Starch Properties and β-Carotene Content in the Storage Root of Sweet Potato (Ipomoea batatas L.)
Published in
Frontiers in Plant Science, March 2016
DOI 10.3389/fpls.2016.00223
Pubmed ID
Authors

Kai Zhang, Zhengdan Wu, Daobin Tang, Changwen Lv, Kai Luo, Yong Zhao, Xun Liu, Yuanxin Huang, Jichun Wang

Abstract

Sweet potato (Ipomoea batatas L.) is a nutritious food crop and, based on the high starch content of its storage root, a potential bioethanol feedstock. Enhancing the nutritional value and starch quantity of storage roots are important goals of sweet potato breeding programs aimed at developing improved varieties for direct consumption, processing, and industrial uses. However, developing improved lines of sweet potato is challenging due to the genetic complexity of this plant and the lack of genome information. Short sequence repeat (SSR) markers are powerful molecular tools for tracking important loci in crops and for molecular-based breeding strategies; however, few SSR markers and marker-trait associations have hitherto been identified in sweet potato. In this study, we identified 1824 SSRs by using a de novo assembly of publicly available ESTs and mRNAs in sweet potato, and designed 1476 primer pairs based on SSR-containing sequences. We mapped 214 pairs of primers in a natural population comprised of 239 germplasms, and identified 1278 alleles with an average of 5.972 alleles per locus and a major allele frequency of 0.7702. Population structure analysis revealed two subpopulations in this panel of germplasms, and phenotypic characterization demonstrated that this panel is suitable for association mapping of starch-related traits. We identified 32, 16, and 17 SSR markers associated with starch content, β-carotene content, and starch composition in the storage root, respectively, using association analysis and further evaluation of a subset of sweet potato genotypes with various characteristics. The SSR markers identified here can be used to select varieties with desired traits and to investigate the genetic mechanism underlying starch and carotenoid formation in the starchy roots of sweet potato.

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Mendeley readers

Mendeley readers

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Geographical breakdown

Country Count As %
Unknown 50 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 22%
Student > Bachelor 6 12%
Student > Master 6 12%
Researcher 5 10%
Student > Doctoral Student 4 8%
Other 6 12%
Unknown 12 24%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 40%
Biochemistry, Genetics and Molecular Biology 8 16%
Pharmacology, Toxicology and Pharmaceutical Science 2 4%
Environmental Science 2 4%
Chemical Engineering 1 2%
Other 2 4%
Unknown 15 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 September 2017.
All research outputs
#3,732,003
of 22,852,911 outputs
Outputs from Frontiers in Plant Science
#1,907
of 20,198 outputs
Outputs of similar age
#58,710
of 298,624 outputs
Outputs of similar age from Frontiers in Plant Science
#46
of 482 outputs
Altmetric has tracked 22,852,911 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 20,198 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 298,624 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 482 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.